EIF2A/CDA02 Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF2A/CDA02 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

64

Reviews (3)

Cat no : 11233-1-AP

Synonyms

EIF2A, EIF2, EIF 2A, EIF, CDA02

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Validation Data Gallery

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  WB analysis of HeLa using 11233-1-AP

  WB result of EIF2A/CDA02 antibody (11233-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HeLa cells.

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  WB analysis using 11233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 11233-1-AP

  IP result of anti-EIF2A/CDA02 (IP:11233-1-AP, 3ug; Detection:11233-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

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  IP experiment of MCF-7 using 11233-1-AP

  IP result of anti-EIF2A/CDA02 (IP:11233-1-AP, 4ug; Detection:11233-1-AP 1:6000) with MCF-7 cells lysate 760 ug.

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  IHC staining of human prostate cancer using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11233-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2A/CDA02 antibody (11233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11233-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11233-1-AP (EIF2A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HepG2 using 11233-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug EIF2A/CDA02 Polyclonal antibody (11233-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293T cells, HeLa cells, PC-12 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human prostate cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Ethacrynic acid treated HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 61 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

11233-1-AP targets EIF2A/CDA02 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF2A/CDA02 fusion protein Ag1753
• Full Name: eukaryotic translation initiation factor 2A, 65kDa
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC011885
• Gene Symbol: EIF2A
• Gene ID (NCBI): 83939
• RRID: AB_2246321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EIF2A, recognizes specifically the 65kd EIF2A protein. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.

Publications

Species	Application	Title
	WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
mouse	WB	Nature; Translation from unconventional 5' start sites drives tumour initiation.; Authors - Ataman Sendoel ; KO Validated
	WB	Science; Translation from the 5' untranslated region shapes the integrated stress response.; Authors - Shelley R Starck ; KD Validated
human	WB	Science; Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.; Authors - Shelley R Starck ; KD Validated
human	WB	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	Sci Adv; CReP mediates selective translation initiation at the endoplasmic reticulum.; Authors - Jonathan P Kastan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gianluca (Verified Customer) (11-25-2021)

Good scientific feedback

• Applications: Western Blot

FH

Daniel (Verified Customer) (04-01-2019)

The provided antibody exceeded all expectations. It worked very well on the first try.Mouse liver was homogenized in Final sample buffer, and then proteins were separated by 10% SDS-PAGE followed by western blot with 11233-1-AP(EIF2A antibody) at a dilution of 1:1000 incubated at 4 degrees Celsius over night in 5% BSA TBST.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Liver

EIF2A/CDA02 Antibody Western Blot, validation (1:1000 dilution) in Mouse Liver (Cat no:11233-1-AP)

FH

Sahya (Verified Customer) (08-13-2018)

Showed a very strong signal, will use at a higher dilution next time.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Skeletal Muscle Satellite Cells