ATF6 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATF6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

276

Reviews (1)

Cat no : 24169-1-AP

Synonyms

cAMP-dependent transcription factor ATF-6 alpha, ATF6-alpha, ATF6A, ATF6 alpha, ATF 6

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Validation Data Gallery

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  WB analysis using 24169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24169-1-AP

  WB result of ATF6 antibody (24169-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 24169-1-AP

  WB result of ATF6 antibody (24169-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HEK-293 cells.

• 

  WB analysis using 24169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24169-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24169-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24169-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24169-1-AP (ATF6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24169-1-AP

  IP result of anti-ATF6 (IP:24169-1-AP, 4ug; Detection:24169-1-AP 1:1500) with HeLa cells lysate 2800ug.

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  IP experiment of HeLa using 24169-1-AP

  IP result of anti-ATF6 (IP:24169-1-AP, 4ug; Detection:24169-1-AP 1:1500) with HeLa cells lysate 680 ug.

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  IHC staining of human cervical cancer using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24169-1-AP (ATF6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24169-1-AP (ATF6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast hyperplasia using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 24169-1-AP (ATF6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24169-1-AP (ATF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24169-1-AP (ATF6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 24169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24169-1-AP (ATF6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 24169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF6 antibody (24169-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 24169-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF6 (24169-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, mouse liver tissue, rat liver, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human breast hyperplasia tissue, human pancreas cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 18 publications below
• WB: See 259 publications below
• IHC: See 16 publications below
• IF: See 18 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

24169-1-AP targets ATF6 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATF6 fusion protein Ag21456
• Full Name: activating transcription factor 6
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 60-70 kDa, 90-100 kDa
• GenBank Accession Number: BC014969
• Gene Symbol: ATF6
• Gene ID (NCBI): 22926
• RRID: AB_2876891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.

Publications

Species	Application	Title
mouse	WB	Cell Stem Cell; Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.; Authors - Changzheng Li
human	WB	J Clin Invest; Flavin-containing monooxygenase 2 confers cardioprotection in ischemia models through its disulfide-bond catalytic activity; Authors - Qingnian Liu
human	IP	J Clin Invest; Ubiquitination of ATF6 by disease-associated RNF186 promotes the innate receptor-induced unfolded protein response.; Authors - Kishu Ranjan
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
mouse	WB	J Clin Invest; Inhibition of the unfolded protein response reduces arrhythmia risk after myocardial infarction.; Authors - Man Liu
human	WB	Circ Res; ATF6 Decreases Myocardial Ischemia/Reperfusion Damage and Links ER Stress and Oxidative Stress Signaling Pathways in the Heart.; Authors - Jung-Kang Jin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Richard (Verified Customer) (11-12-2019)

The antibody works well and gives two clean bands for cleaved and uncleaved ATF6 by western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HeLa, HEK293T

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