XBP1S/XBP1U Polyclonal antibody

• Featured Product
• KD/KO Validated

XBP1S/XBP1U Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, FC (Intra), ChIP, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 24168-1-AP

Synonyms

XBP1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 24168-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24168-1-AP (XBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24168-1-AP

  WB result of XBP1S/XBP1U antibody (24168-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-XBP1S/XBP1U transfected HepG2 cells.

• 

  WB analysis using 24168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24168-1-AP (XBP1S/XBP1U antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24168-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24168-1-AP (XBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24168-1-AP (XBP1S/XBP1U antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 24168-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XBP1S/XBP1U (24168-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, Jurkat cells, Raji cells
• Positive IHC detected in: human breast cancer tissue, mouse liver tissue, human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• ChIP: See 1 publications below

Product Information

24168-1-AP targets XBP1S/XBP1U in WB, IHC, FC (Intra), ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XBP1S/XBP1U fusion protein Ag21454
• Full Name: X-box binding protein 1
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: ~32 kDa; ~60 kDa
• GenBank Accession Number: BC000938
• Gene Symbol: XBP1
• Gene ID (NCBI): 7494
• RRID: AB_2879445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124). It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum, the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1. The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1S, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1U, is constitutively expressed, and thought to function as a negative feedback regulator of XBP1S, which shuts off transcription of target genes during the recovery phase of ER stress (PMID: 11850408). The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The XBP1 antibody (24168-1-AP) can detect both XBP1U and XBP1S.

Publications

Species	Application	Title
rat	WB,IHC	J Hazard Mater; GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline; Authors - Li Mou
human	WB	J Ethnopharmacol; Network pharmacology, molecular docking, and in vitro experimental verification of the mechanism of Guanxining in treating diabetic atherosclerosis; Authors - Wenting Wang
mouse	WB	Bioorg Chem; Dihydroartemisinin alleviates steatosis and inflammation in nonalcoholic steatohepatitis by decreasing endoplasmic reticulum stress and oxidative stress.; Authors - Tongxi Li
human	WB	J Virol; Inositol requiring enzyme 1α promotes Zika virus infection through regulating stearoyl-CoA desaturase 1 mediated lipid metabolism.; Authors - Yanxia Huang ; KD Validated
mouse	WB	Exp Ther Med; Apelin‑13 reduces high glucose‑induced mitochondrial dysfunction in cochlear hair cells by inhibiting endoplasmic reticulum stress; Authors - Zhiqiang Huo
mouse	WB	J Zhejiang Univ Sci B; Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure; Authors - Xue YANG

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

• Applications: Other,