• Überblick
  • Geprüfte Anwendungen
  • Produktinformation
  • Publikationen (103)
  • Protokolle
  • Rezensionen (1)
  • Verwandte Produkte (8)

VWF, VWFpp Polyklonaler Antikörper

VWF, VWFpp Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11778-1-AP

Synonyme

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF, VWF, VWFpp



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHUVEC-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Mausmilzgewebe, Rattenmilzgewebe
Erfolgreiche IPMausmilzgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 31 publications below

Produktinformation

11778-1-AP bindet in WB, IP, ELISA VWF, VWFpp und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Hausschwein, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen VWF, VWFpp fusion protein Ag2386
Vollständiger Name von Willebrand factor
Berechnetes Molekulargewicht 309 kDa
Beobachtetes Molekulargewicht 309-320 kDa, 75-100 kDa
GenBank-Zugangsnummer.
Gene symbol VWF
Gene ID (NCBI) 7450
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Von Willebrand factor (vWF) is a large multimeric glycoprotein that is crucial to the hemostasis process. It supports platelet adhesion and carries factor VIII (FVIII). Deficiency of vWF results in von Willebrand disease (vWD), a common inherited bleeding disorder. The pre-pro-vWF is comprised of 2,813 amino acids (aa) which encompass a 22-aa signal peptide, a 741-aa large propeptide (vWF propeptide, vWFpp, also called von Willebrand antigen 2) and 2,050 aa making up the mature vWF. The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. This antibody raised against the N-terminal region of pre-pro-vWF recognizes von Willebrand antigen 2 (75-100 kDa) and pro-vWF (309-320 kDa). (PMID: 9759493; 12067899; 22452980; 8874191)

Protokolle

Produktspezifische Protokolle
WB protocol for VWF, VWFpp antibody 11778-1-APProtokoll herunterladen
IP protocol for VWF, VWFpp antibody 11778-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
mouse

J Thromb Haemost

Knock-in mice bearing constitutively active αIIb(R990W) mutation develop macrothrombocytopenia with severe platelet dysfunction.

Authors - Keigo Akuta
mouse

Circulation

Hyperglycemia inhibits cardiac stem cell-mediated cardiac repair and angiogenic capacity.

Authors - André S D Molgat
pig

Circulation

Intramyocardial peptide nanofiber injection improves postinfarction ventricular remodeling and efficacy of bone marrow cell therapy in pigs.

Authors - Lin Yi-Dong YD
mouse

Acta Pharm Sin B

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

Authors - Weimin Yu
rat

Adv Healthc Mater

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Authors - Zhi Zheng
rat

ACS Appl Mater Interfaces

Aligned Graphene Mesh-Supported Double Network Natural Hydrogel Conduit Loaded with Netrin-1 for Peripheral Nerve Regeneration.

Authors - Qun Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kim-Anh (Verified Customer) (09-03-2019)

Good primary antibody, I used for IF test on HUVECs

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: huvecs

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