F8 Polyclonal antibody

F8 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21485-1-AP

Synonyms

AHF, Antihemophilic factor, Coagulation factor VIII, DXS1253E, F8, F8B, F8C, FVIII, HEMA, Procoagulant component

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Validation Data Gallery

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  IF Staining of human umbilical vein endothelial cells using 21485-1-AP

  IF result of anti-F8 (21485-1-AP) with primary human umbilical vein endothelial cells.

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  IF Staining of HUVEC using 21485-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 21485-1-AP (F8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IF/ICC detected in: human umbilical vein endothelial cells, HUVEC cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : N/A

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 21485-1-AP is F8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: F8 fusion protein Ag15772
• Full Name: coagulation factor VIII, procoagulant component
• Calculated Molecular Weight: 2351 aa, 267 kDa
• GenBank Accession Number: BC022513
• Gene Symbol: F8
• Gene ID (NCBI): 2157
• RRID: AB_2878868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

F8, also named as AHF and F8C, belongs to the multicopper oxidase family. F8, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. F8 can be cleaved to 4 chains: F8a heavy chain, 200 kDa isoform, F8a heavy chain, 92 kDa isoform, F8 B chain and F8a light chain. This antibody can recognize the light chain.

Publications

Species	Application	Title
human	IF	Invest Ophthalmol Vis Sci; miR-590-3p Inhibits Pyroptosis in Diabetic Retinopathy by Targeting NLRP1 and Inactivating the NOX4 Signaling Pathway.; Authors - Chufeng Gu