P-selectin / CD62P Polyclonal antibody

P-selectin / CD62P Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13304-1-AP

Synonyms

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP



Tested Applications

Positive WB detected inHuman peripheral blood platelets
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13304-1-AP targets P-selectin / CD62P in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P-selectin / CD62P fusion protein Ag4124
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Calculated Molecular Weight 830 aa, 91 kDa
GenBank Accession NumberBC028067
Gene Symbol SELP
Gene ID (NCBI) 6403
RRIDAB_2877937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Protocols

Product Specific Protocols
WB protocol for P-selectin / CD62P antibody 13304-1-APDownload protocol
IHC protocol for P-selectin / CD62P antibody 13304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Appl Mater Interfaces

AIEgen-Based Bionic Nanozymes for the Interventional Photodynamic Therapy-Based Treatment of Orthotopic Colon Cancer.

Authors - Yanhong Duo
humanWB

Int J Biol Macromol

Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing

Authors - Hang Dong
humanFC

World J Gastroenterol

Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer

Authors - Jia-Cheng Li
humanIF,FC

Immun Inflamm Dis

Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis.

Authors - Shihua Zhang
humanWB

Nat Commun

Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions

Authors - Chinnaswamy Tiruppathi
WB

Small

Platelet Membrane Coated Cu9S8-SNAP for Targeting NIR-II Mild Photothermal Enhanced Chemodynamic/Gas Therapy of Triple-Negative Breast Cancer

Authors - Danping Wu