CD42a / GP9 Polyclonal antibody

CD42a / GP9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14564-1-AP

Synonyms

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX

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Validation Data Gallery

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  WB analysis of human peripheral blood platelets using 14564-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 14564-1-AP (CD42a / GP9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 14564-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 14564-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Human peripheral blood platelets
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

14564-1-AP targets CD42a / GP9 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD42a / GP9 fusion protein Ag6129
• Full Name: glycoprotein IX (platelet)
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC030229
• Gene Symbol: CD42a
• Gene ID (NCBI): 2815
• RRID: AB_2878067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.

Publications

Species	Application	Title
mouse	WB,IF	Cell Death Dis; Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression; Authors - Rong Yan