P-selectin / CD62P Monoclonal antibody
P-selectin / CD62P Monoclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3G2C7
Cat no : 60322-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human peripheral blood platelets, Jurkat cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 14 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| FC | See 3 publications below |
Product Information
60322-1-Ig targets P-selectin / CD62P in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | P-selectin / CD62P fusion protein Ag4124 |
| Full Name | selectin P (granule membrane protein 140kDa, antigen CD62) |
| Calculated molecular weight | 830 aa, 91 kDa |
| Observed molecular weight | 140 kDa, 105-110 kDa |
| GenBank accession number | BC028067 |
| Gene symbol | SELP |
| Gene ID (NCBI) | 6403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P-selectin / CD62P antibody 60322-1-Ig | Download protocol |
| IHC protocol for P-selectin / CD62P antibody 60322-1-Ig | Download protocol |
| IP protocol for P-selectin / CD62P antibody 60322-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Biomater Rescuing ischemic stroke by biomimetic nanovesicles through accelerated thrombolysis and sequential ischemia-reperfusion protection. | ||
Front Oncol Platelet Membranes Coated Gold Nanocages for Tumor Targeted Drug Delivery and Amplificated Low-Dose Radiotherapy. | ||
Commun Biol Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells. | ||
Front Bioeng Biotechnol Nano-Platelets as an Oxygen Regulator for Augmenting Starvation Therapy Against Hypoxic Tumor. | ||
Biol Res CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis. |
