CD31 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD31 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

375

Cat no : 11265-1-AP

Synonyms

PECAM1, PECAM 1, PECA1, GPIIA', GPIIA

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Validation Data Gallery

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  WB analysis using 11265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11265-1-AP (CD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11265-1-AP (CD31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 11265-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 11265-1-AP (CD31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 11265-1-AP

  IP result of anti-CD31 (IP:11265-1-AP, 5ug; Detection:11265-1-AP 1:300) with Jurkat cells lysate 4000ug.

• 

  IHC staining of human liver cancer using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human tonsillitis using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11265-1-AP (CD31 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11265-1-AP (CD31 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IF Staining of human endometrial cancer using 11265-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using CD31 antibody (11265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human endometrial cancer using 11265-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using CD31 antibody (11265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 11265-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 11265-1-AP (CD31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 11265-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CD31 antibody (11265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human peripheral blood platelets, HUVEC cells, Jurkat cells, THP-1 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human tonsillitis tissue, human placenta tissue, human renal cell carcinoma tissue, human hepatocirrhosis tissue, human kidney tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human endometrial cancer tissue, human tonsillitis tissue
• Positive IF/ICC detected in: HUVEC cells, human tonsillitis tissue, human endometrial cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 89 publications below
• IHC: See 155 publications below
• IF: See 173 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Product Information

11265-1-AP targets CD31 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, rabbit, canine, monkey, zebrafish, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD31 fusion protein Ag1787
• Full Name: platelet/endothelial cell adhesion molecule
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC022512
• Gene Symbol: CD31
• Gene ID (NCBI): 5175
• RRID: AB_2299349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
	IHC	Bioact Mater; Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration; Authors - Yiting Sun
rabbit	WB,IF	Nat Commun; Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction; Authors - Yujie Hua
human	IHC	Sci Transl Med; Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.; Authors - Kana Hasegawa
human	IHC,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	IHC	Mol Cancer; CircPTPRA blocks the recognition of RNA N6-methyladenosine through interacting with IGF2BP1 to suppress bladder cancer progression.; Authors - Fei Xie
monkey,human	IHC	Acta Pharm Sin B; Nonclinical safety, tolerance and pharmacodynamics evaluation for meplazumab treating chloroquine-resistant Plasmodium falciparum.; Authors - Kun Zhang

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