CD42b Polyclonal antibody

CD42b Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 12860-1-AP

Synonyms

BSS, CD42b, CD42b alpha, GP1B, GP1BA

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Validation Data Gallery

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  WB analysis of human peripheral blood platelets using 12860-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 12860-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig peripheral blood leukocyte using 12860-1-AP

  pig peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12860-1-AP (CD42b antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12860-1-AP (CD42b antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12860-1-AP (CD42b antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12860-1-AP (CD42b antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12860-1-AP (CD42b antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 12860-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12860-1-AP (CD42b antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Human peripheral blood platelets, pig peripheral blood leukocyte, human blood
• Positive IHC detected in: human spleen tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 11 publications below
• IF: See 2 publications below

Product Information

12860-1-AP targets CD42b in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD42b fusion protein Ag3893
• Full Name: glycoprotein Ib (platelet), alpha polypeptide
• Calculated Molecular Weight: 626 aa, 69 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC027955
• Gene Symbol: CD42b
• Gene ID (NCBI): 2811
• RRID: AB_10644481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GP1BA, also named as CD42b , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

Publications

Species	Application	Title
human	WB	Adv Healthc Mater; Platelet Membrane-Fused Circulating Extracellular Vesicles Protect the Heart from Ischemia/Reperfusion Injury; Authors - Jizong Jiang
mouse	IF	Cancer Lett; Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma; Authors - Qianyu Shi
rat	WB	Theranostics; A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.; Authors - Peipei Jin
human	WB	Int J Biol Macromol; Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing; Authors - Hang Dong
mouse	WB	ACS Biomater Sci Eng; Biomimetic Platform Based on Mesoporous Platinum for Multisynergistic Cancer Therapy.; Authors - Gaoqian Zhao
mouse	IHC	Biochem Pharmacol; GSK669, a NOD2 receptor antagonist, inhibits thrombosis and oxidative stress via targeting platelet GPVI.; Authors - Guanxing Pan

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