TIMP1 Polyklonaler Antikörper

TIMP1 Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Kat-Nr. : 10753-1-AP

Synonyme

CLGI, Collagenase inhibitor, EPA, HCI, Metalloproteinase inhibitor 1, TIMP, TIMP 1, TIMP1, TIMP-1

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 10753-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10753-1-AP (TIMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10753-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10753-1-AP (TIMP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10753-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10753-1-AP (TIMP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10753-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10753-1-AP (TIMP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 8 publications below
• IF: See 1 publications below

Produktinformation

10753-1-AP bindet in IF, IHC, ELISA TIMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIMP1 fusion protein Ag1146
• Vollständiger Name: TIMP metallopeptidase inhibitor 1
• Berechnetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC007097
• Gene symbol: TIMP1
• Gene ID (NCBI): 7076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tissue Inhibitor of Metalloproteinases-1 (TIMP1) is a naturally occurring protein in the human organism regulating the activity of matrix metalloproteinases (MMPs). TIMP1 encodes a 931 base-pair mRNA and a 207 amino acid protein. Overexpression of TIMP1 can lead to a substantially increase of genes involved in proliferation, apoptosis and signal transduction. In addition, TIMP1 was found to decrease tumor cell sensitivity to multiple anticancer drugs by activation of downstream pathways and exhibited anti-apoptotic activity. Specifically, the TIMP1 could degrade cyclinB1 and activate the NF-kβ signaling pathway to protect breast cancer cells against chemotherapy-induced cell death.

Publikationen

Species	Application	Title
mouse		Int J Biol Macromol; Anti-metastatic effects of antrodan, the Antrodia cinnamomea mycelia glycoprotein, in lung carcinoma cells.; Authors - Kuan-Ning Fa
human	IHC	Br J Pharmacol; Dual Inhibition of Cannabinoid-1 Receptor and iNOS Attenuates Obesity-induced Chronic Kidney Disease.; Authors - Shiran Udi
mouse	IHC	Sci Rep; FOXO transcription factors protect against the diet-induced fatty liver disease.; Authors - Xiaoyan Pan
mouse		Sci Rep; Quercetin prevents hepatic fibrosis by inhibiting hepatic stellate cell activation and reducing autophagy via the TGF-β1/Smads and PI3K/Akt pathways.; Authors - Liwei Wu
human		Mar Drugs; 11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways.; Authors - Jen-Jie Lin
mouse		Mediators Inflamm; Protective Effect of Astaxanthin on Liver Fibrosis through Modulation of TGF-β1 Expression and Autophagy.; Authors - Miao Shen

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