MMP7 Polyclonal antibody

• Featured Product
• KD/KO Validated

MMP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

89

Cat no : 10374-2-AP

Synonyms

MMP 7, Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin

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Validation Data Gallery

• 

  WB analysis using 10374-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10374-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10374-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10374-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10374-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas cancer using 10374-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas cancer using 10374-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-3 using 10374-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MMP7 antibody (10374-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, human placenta tissue, SKOV-3 cells, NIH/3T3 cells, COLO 320 cells, PC-3 cells
• Positive IHC detected in: human pancreas cancer tissue, human prostate cancer tissue, human colon cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human pancreas cancer tissue
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 74 publications below
• IHC: See 18 publications below
• IF: See 1 publications below

Product Information

10374-2-AP targets MMP7 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP7 fusion protein Ag0550
• Full Name: matrix metallopeptidase 7 (matrilysin, uterine)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC003635
• Gene Symbol: MMP7
• Gene ID (NCBI): 4316
• RRID: AB_2144452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Matrix metalloproteinase-7 (MMP-7)/ matrilysin is a member of the MMP family, but is structurally different from the other MMPs by virtue of the absence of a conserved COOH-terminal protein domain. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and cancer metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-7 degrades proteoglycans, fibronectin, elastin and casein, and is involved in wound healing, tumor progression, pulmonary fibrosis, and development of choroidal neovascularization in age-related macular degeneration. The expression of MMP-7 is increased in most tumors. This antibody can only recognize the full-length of MMP7.

Publications

Species	Application	Title
human	WB	Cancer Res; Hypoxic tumor-derived exosomal miR-301a mediates M2 macrophage polarization via PTEN/PI3Kγ to promote pancreatic cancer metastasis.; Authors - Xiaofeng Wang
human	WB	Cancer Res; Ubiquitin-like protein FAT10 promotes the invasion and metastasis of hepatocellular carcinoma by modifying β-catenin degradation.; Authors - Rongfa Yuan
human	IHC	Cancer Res; KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.; Authors - Peng Yuan
human	WB	Oncogene; Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.; Authors - Q Zhou
human	WB	Clin Cancer Res; BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs.; Authors - Zebing Liu
human,mouse	WB,IHC	Int J Cancer; Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.; Authors - Qingmin Yuan

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