Tested Applications
| Positive WB detected in | A2780 cells, A549 cells, HeLa cells, human skeletal muscle tissue, U-87 MG cells, PC-12 cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17873-1-AP targets MMP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12359 Product name: Recombinant human MMP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-477 aa of BC074869 Sequence: GEDTSMNLVQKYLENYYDLEKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC Predict reactive species |
| Full Name | matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| Calculated Molecular Weight | 477 aa, 54 kDa |
| Observed Molecular Weight | 45-60 kDa |
| GenBank Accession Number | BC074869 |
| Gene Symbol | MMP3 |
| Gene ID (NCBI) | 4314 |
| RRID | AB_2146587 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08254 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MMP3 antibody 17873-1-AP | Download protocol |
| WB protocol for MMP3 antibody 17873-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MMP3 polyclonal antibody (Cat# 17873-1-AP) has accumulated 348 citations. Publications appear in high-impact journals including Cell, Nature Genetics, Nature Communications, Bioactive Materials, Bone Research, Advanced Science, Cell Death & Disease, and International Immunopharmacology. Research fields span osteoarthritis, intervertebral disc degeneration, rheumatoid arthritis, ferroptosis, cellular senescence, inflammation, and multiple cancer types (breast, gastric, colorectal, pancreatic, glioma).
| Species | Application | Title |
|---|---|---|
Nat Nanotechnol A disease-severity-responsive nanoparticle enables potent ghrelin messenger RNA therapy in osteoarthritis. | ||
Exploration (Beijing) Peptide RL-QN15 Regulates Functions of Epidermal Stem Cells to Accelerate Skin Wound Regeneration via the FZD8/β-Catenin Axis. | ||
Mil Med Res D-mannose alleviates intervertebral disc degeneration through glutamine metabolism | ||
Bioact Mater In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones. |
Reviews
What customers say
One user rated this product 5 out of 5 stars. They used it for immunofluorescence on intervertebral disc cells at a 1:100 dilution (lot 00082776). Their protocol included permeabilization with 0.3% Triton X-100 and blocking with 2.5% BSA plus 0.1% Triton X-100. The review was posted in July 2022.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ana Luísa (Verified Customer) (07-25-2022) | Permeabilization: 0,3% Triton X-100 Blocking 2,5% BSA + 0,1% Triton X-100
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