Tested Applications
| Positive WB detected in | A2780 cells, A549 cells, HeLa cells, human skeletal muscle tissue, U-87 MG cells, PC-12 cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17873-1-AP targets MMP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12359 Product name: Recombinant human MMP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-477 aa of BC074869 Sequence: GEDTSMNLVQKYLENYYDLEKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC Predict reactive species |
| Full Name | matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| Calculated Molecular Weight | 477 aa, 54 kDa |
| Observed Molecular Weight | 45-60 kDa |
| GenBank Accession Number | BC074869 |
| Gene Symbol | MMP3 |
| Gene ID (NCBI) | 4314 |
| RRID | AB_2146587 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08254 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MMP3 antibody 17873-1-AP | Download protocol |
| WB protocol for MMP3 antibody 17873-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-MMP3 antibody (Cat# 17873-1-AP) has accumulated 348 citations published in high-impact journals including Nature Genetics, Nature Communications, Cell, Bioactive Materials, Bone Research, Advanced Science, Phytomedicine, International Immunopharmacology, Osteoarthritis and Cartilage, and Journal of Nanobiotechnology. Associated research fields encompass osteoarthritis, intervertebral disc degeneration, rheumatoid arthritis, cancer biology, ferroptosis, cellular senescence, inflammation, wound healing, diabetes, and stroke.
| Species | Application | Title |
|---|---|---|
Nat Nanotechnol A disease-severity-responsive nanoparticle enables potent ghrelin messenger RNA therapy in osteoarthritis. | ||
Exploration (Beijing) Peptide RL-QN15 Regulates Functions of Epidermal Stem Cells to Accelerate Skin Wound Regeneration via the FZD8/β-Catenin Axis. | ||
Mil Med Res D-mannose alleviates intervertebral disc degeneration through glutamine metabolism | ||
Bioact Mater Microenvironment-responsive metal-phenolic network release platform with ROS scavenging, anti-pyroptosis, and ECM regeneration for intervertebral disc degeneration |
Reviews
What customers say
The single review for 17873-1-AP is from Ana Luísa Castro (July 2022), who rated it 5 stars. She used it for immunofluorescence on intervertebral disc cells at a 1:100 dilution. Her protocol notes include 0.3% Triton X-100 for permeabilization and 2.5% BSA with 0.1% Triton X-100 for blocking. Overall, the reviewer had a positive experience with the product.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ana Luísa (Verified Customer) (07-25-2022) | Permeabilization: 0,3% Triton X-100 Blocking 2,5% BSA + 0,1% Triton X-100
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