MMP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MMP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

202

Reviews (1)

Cat no : 10371-2-AP

Synonyms

Interstitial collagenase, EC:3.4.24.7, CLGN, CLG, 27 kDa interstitial collagenase

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Validation Data Gallery

• 

  WB analysis of U-87 MG using 10371-2-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 10371-2-AP (MMP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 cells using 10371-2-AP

  WB results of MMP1 antibody (10371-1-AP, 1:500) with NIH3T3 cells, 62 kDa for pro-MMP1 and 54 kDa for Active MMP1. Data from Dr. Hui-Wen Chiu, Taipei Medical University.

• 

  WB analysis using 10371-2-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 10371-2-AP (MMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 10371-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10371-2-AP (MMP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10371-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP1 antibody (10371-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10371-2-AP

  Immunofluorescent analysis of HepG2 cells, using MMP1 antibody 10371-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: U-87 MG cells, NIH/3T3 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 167 publications below
• IHC: See 38 publications below
• IF: See 4 publications below

Product Information

10371-2-AP targets MMP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP1 fusion protein Ag0547
• Full Name: matrix metallopeptidase 1 (interstitial collagenase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 45-48 kDa；55-62 kDa
• GenBank Accession Number: BC013875
• Gene Symbol: MMP1
• Gene ID (NCBI): 4312
• RRID: AB_2297741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

Publications

Species	Application	Title
human	WB	Cell Res; Identification of TRA2B-DNAH5 fusion as a novel oncogenic driver in human lung squamous cell carcinoma.; Authors - Fei Li
human	IF	Biomaterials; Material-driven immunomodulation and ECM remodeling reverse pulmonary fibrosis by local delivery of stem cell-laden microcapsules; Authors - Yujie Zhang
human	IF	Biomaterials; Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.; Authors - Angelina D Schoenenberger
rat	WB	Acta Biomater; ROS-responsive magnesium-containing microspheres for antioxidative treatment of intervertebral disc degeneration; Authors - Tianhui Zhang
human	IHC	Br J Cancer; Chemerin suppresses hepatocellular carcinoma metastasis through CMKLR1-PTEN-Akt axis.; Authors - Jing-Jing Li
rat	WB,IF	Bioact Mater; Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.; Authors - Liwen Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Saravanakumar (Verified Customer) (10-31-2017)

I highly recommencement this (MMP1) antibody works good for me in MCF10A breast normal epithelial cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF10A cells

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