Stathmin 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Stathmin 1 Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

25

Rezensionen (1)

Kat-Nr. : 11157-1-AP

Synonyme

STMN1, stathmin 1/oncoprotein 18, Stathmin, OP18, Leukemia-associated phosphoprotein p18

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11157-1-AP

  WB results of Stathmin antibody (11157-1-AP, 1:20000) with si-control and si-Stathmin transfected Jurkat cells..

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  WB analysis using 11157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11157-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11157-1-AP (Stathmin 1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 11157-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Stathmin 1 (11157-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 8 publications below
• IF: See 3 publications below

Produktinformation

11157-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA Stathmin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Stathmin 1 fusion protein Ag1633
• Vollständiger Name: stathmin 1/oncoprotein 18
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC014353
• Gene symbol: Stathmin 1
• Gene ID (NCBI): 3925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

Publikationen

Species	Application	Title
mouse	WB,IHC	Cell Stem Cell; Continuous clonal labeling reveals uniform progenitor potential in the adult exocrine pancreas.; Authors - Sophie C Lodestijn
rat	WB	Chemosphere; Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.; Authors - Mingdan You
human	WB	Int J Nanomedicine; Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.; Authors - Chen Li
human	WB	Oncogene; KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.; Authors - Lei Zhao ; KD Validated
human	IHC	Oncotarget; Stathmin and phospho-stathmin protein signature is associated with survival outcomes of breast cancer patients.; Authors - Xia-Ying Kuang
human	IHC	Neuroimage; Developmental dynamics of the periventricular parietal crossroads of growing cortical pathways in the fetal brain - In vivo fetal MRI with histological correlation.; Authors - Ruxandra-Iulia Milos

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Richard (Verified Customer) (10-08-2019)

Antibody works well at 1:4000 and gives a single band, have no issues with it.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: HeLa

Stathmin 1 Antibody Western Blot, validation (1:4000 dilution) in HeLa (Cat no:11157-1-AP)

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