Uteroglobin/CC10 Polyklonaler Antikörper

Uteroglobin/CC10 Polyklonal Antikörper für IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (1)

Kat-Nr. : 10490-1-AP

Synonyme

SCGB1A1, Uteroglobin, Club cells 10 kDa secretory protein, Club cell phospholipid-binding protein, CCSP

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Galerie der Validierungsdaten

• 

  IHC staining of human lung cancer using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse lung using 10490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Uteroglobin/CC10 antibody (10490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 10490-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (10490-1-AP) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Lungenkarzinomgewebe, Rattenlungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslungengewebe
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 9 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• FC: See 3 publications below

Produktinformation

10490-1-AP bindet in WB, IHC, IF-P, FC (Intra), IP, ELISA Uteroglobin/CC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Uteroglobin/CC10 fusion protein Ag0758
• Vollständiger Name: secretoglobin, family 1A, member 1 (uteroglobin)
• Berechnetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC004481
• Gene symbol: Uteroglobin
• Gene ID (NCBI): 7356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

Publikationen

Species	Application	Title
human	IHC	Nature; SARS-CoV-2 Omicron variant replication in human bronchus and lung ex vivo.; Authors - Kenrie P Y Hui
human	IHC	Lancet Respir Med; Tropism, replication competence, and innate immune responses of the coronavirus SARS-CoV-2 in human respiratory tract and conjunctiva: an analysis in ex-vivo and in-vitro cultures.; Authors - Kenrie P Y Hui
human	IHC	Theranostics; A SCID mouse-human lung xenograft model of SARS-CoV-2 infection.; Authors - Wenkun Fu
human	IF	PLoS Pathog; Lung adenocarcinoma originates from retrovirus infection of proliferating type 2 pneumocytes during pulmonary post-natal development or tissue repair.; Authors - Murgia Claudio C
mouse	IHC	J Pathol; Overexpression of the SARS-CoV-2 receptor ACE2 is induced by cigarette smoke in bronchial and alveolar epithelia.; Authors - Aibin Liu
human	WB	Antioxidants (Basel); Empagliflozin-Enhanced Antioxidant Defense Attenuates Lipotoxicity and Protects Hepatocytes by Promoting FoxO3a- and Nrf2-Mediated Nuclear Translocation via the CAMKK2/AMPK Pathway.; Authors - Yangyang Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tessa (Verified Customer) (02-03-2019)

Used in 20pcw human fetal lung and got nice staining. Looks very specific with low background. Found that it did not require antigen retrieval in cryosections. In fact it turned out better in sections where 10mM Na Citrate buffer at pH6 was not used.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Fetal Lung

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