CD14 Monoclonal antibody

CD14 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse and More (1)


WB, IF, IHC, ELISA, Cell treatment





Cat no : 60253-1-Ig


CD14, CD14 molecule

Tested Applications

Positive WB detected inTHP-1 cells, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells, human tonsillitis tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60253-1-Ig is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD14 fusion protein Ag10693
Full Name CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.


Product Specific Protocols
WB protocol for CD14 antibody 60253-1-IgDownload protocol
IHC protocol for CD14 antibody 60253-1-IgDownload protocol
IF protocol for CD14 antibody 60253-1-IgDownload protocol
FC protocol for CD14 antibody 60253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Front Pharmacol

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sareena (Verified Customer) (06-06-2023)

Works well when conjugated to fluorophores and tested with compensation beads

  • Applications: Immunofluorescence, Flow Cytometry
  • Primary Antibody Dilution: CD14 Monoclonal antibody

Sunil (Verified Customer) (05-15-2023)

Great and fast delivery which is always useful!

  • Applications: Flow Cytometry

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Tris-EDTA antigen retrieval pH 9.0.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
CD14 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:60253-1-Ig)