CD14 Monoclonal antibody
CD14 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IF-P, ELISA, Cell treatment
Conjugate
Unconjugated
CloneNo.
2C1D9
Cat no : 60253-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, human milk, human placenta tissue, RAW 264.7 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | RAW 264.7 cells, human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 5 publications below |
| IF | See 6 publications below |
| FC | See 1 publications below |
Product Information
60253-1-Ig targets CD14 in WB, IHC, IF/ICC, IF-P, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD14 fusion protein Ag10693 |
| Full Name | CD14 molecule |
| Calculated Molecular Weight | 375 aa, 40 kDa |
| Observed Molecular Weight | 45-55 kDa |
| GenBank Accession Number | BC010507 |
| Gene Symbol | CD14 |
| Gene ID (NCBI) | 929 |
| RRID | AB_2881374 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD14 antibody 60253-1-Ig | Download protocol |
| IHC protocol for CD14 antibody 60253-1-Ig | Download protocol |
| IF protocol for CD14 antibody 60253-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells. | ||
Arthritis Rheumatol Attenuation of rheumatoid arthritis through the inhibition of caspase3/GSDME-mediated pyroptosis induced by TNF-α. | ||
Bioact Mater Novel magnetic silk fibroin scaffolds with delayed degradation for potential long-distance vascular repair. | ||
Sci Rep High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage. | ||
Front Pharmacol Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway | ||
J Cancer MMP9 and IGFBP1 Regulate Tumor Immune and Drive Tumor Progression in Clear Cell Renal Cell Carcinoma. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sareena (Verified Customer) (06-06-2023) | Works well when conjugated to fluorophores and tested with compensation beads
|
FH Sunil (Verified Customer) (05-15-2023) | Great and fast delivery which is always useful!
|
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue. Tris-EDTA antigen retrieval pH 9.0.
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