PTGDS Polyclonal antibody

PTGDS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10754-2-AP

Synonyms

LPGDS, Lipocalin-type prostaglandin-D synthase, Glutathione-independent PGD synthase, EC:5.3.99.2, Cerebrin-28

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Validation Data Gallery

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  WB analysis of HeLa using 10754-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10754-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 10754-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10754-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 10754-2-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10754-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human kidney tissue, human plasma, human heart tissue, human colon tissue, PC-3 cells, human urine tissue
• Positive IHC detected in: mouse brain tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 3 publications below

Product Information

10754-2-AP targets PTGDS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTGDS fusion protein Ag1163
• Full Name: prostaglandin D2 synthase 21kDa (brain)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC005939
• Gene Symbol: PTGDS
• Gene ID (NCBI): 5730
• RRID: AB_2174610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycoprotein prostaglandin D2 synthase (PTGDS), also known as L-PGDS, PGDS, and LPGDS, is a member of the lipocalin superfamily and plays dual roles in prostaglandins metabolism and lipid transport (PMID: 34743203). PTGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PTGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. PTGDS is abundantly expressed in the brain and heart and has also been found in testis, prostate, kidney, spleen and leukocytes. It can be found as a collection of monomeric glycoforms with molecular weights in the 27-34 kDa range (PMID: 32430846).

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway.; Authors - Hui-Yuan Su
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu
human	WB	Cell Cycle; MicroRNA-138 improves LPS-induced trophoblast dysfunction through targeting RELA and NF-κB signaling.; Authors - Ailan Yin
human	IHC	Cancer Manag Res; Decreased PTGDS Expression Predicting Poor Survival of Endometrial Cancer by Integrating Weighted Gene Co-Expression Network Analysis and Immunohistochemical Validation.; Authors - Ruoyao Zou
human	WB	Med Sci Monit; Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma.; Authors - Kim Jae Ho JH
mouse	IHC	Mol Neurodegener; MLKL deficiency alleviates neuroinflammation and motor deficits in the α-synuclein transgenic mouse model of Parkinson's disease; Authors - Lu Geng