PTGDS Polyclonal antibody

PTGDS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10754-2-AP

Synonyms

Beta trace protein, Cerebrin 28, LPGDS, PDS, PGD2, PGD2 synthase, PGDS, PGDS2, Prostaglandin D2 synthase, Prostaglandin H2 D isomerase, PTGDS

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Validation Data Gallery

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  WB analysis of HeLa using 10754-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10754-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10754-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 10754-2-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10754-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 10754-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human kidney tissue, human plasma, human heart tissue, human colon tissue, PC-3 cells, human urine tissue
• Positive IHC detected in: mouse brain tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 3 publications below

Product Information

10754-2-AP targets PTGDS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTGDS fusion protein Ag1163
• Full Name: prostaglandin D2 synthase 21kDa (brain)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC005939
• Gene Symbol: PTGDS
• Gene ID (NCBI): 5730
• RRID: AB_2174610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGDS(Prostaglandin-H2 D-isomerase) is also named as PTGDS, PDS, PGDS2. It catalyzes the conversion of PGH2 to PGD2, and is a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. It has two N-glycosylation recognition sites.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway.; Authors - Hui-Yuan Su
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu
human	WB	Cell Cycle; MicroRNA-138 improves LPS-induced trophoblast dysfunction through targeting RELA and NF-κB signaling.; Authors - Ailan Yin
human	IHC	Cancer Manag Res; Decreased PTGDS Expression Predicting Poor Survival of Endometrial Cancer by Integrating Weighted Gene Co-Expression Network Analysis and Immunohistochemical Validation.; Authors - Ruoyao Zou
human	WB	Med Sci Monit; Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma.; Authors - Kim Jae Ho JH
mouse	IHC	Mol Neurodegener; MLKL deficiency alleviates neuroinflammation and motor deficits in the α-synuclein transgenic mouse model of Parkinson's disease; Authors - Lu Geng