Fibronectin Polyclonal antibody

• Featured Product
• KD/KO Validated

Fibronectin Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

675

Reviews (8)

Cat no : 15613-1-AP

Synonyms

FN1, fibronectin 1, ED B, Cold-insoluble globulin, Cold insoluble globulin

---

Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 15613-1-AP

  WB result of Fibronectin antibody (15613-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fibronectin transfected NIH/3T3 cells.

• 

  WB analysis of human plasma using 15613-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 15613-1-AP (Fibronectin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15613-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15613-1-AP (Fibronectin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 15613-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15613-1-AP (Fibronectin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 15613-1-AP

  IP result of anti-Fibronectin (IP:15613-1-AP, 4ug; Detection:15613-1-AP 1:3000) with NIH/3T3 cells lysate 1680 ug.

• 

  IHC staining of human colon using 15613-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15613-1-AP (Fibronectin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 15613-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (15613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of NIH/3T3 using 15613-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (15613-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of NIH/3T3 using 15613-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (15613-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 15613-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 15613-1-AP (Fibronectin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 15613-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human Fibronectin (15613-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: human plasma, NIH/3T3 cells, MDA-MB-453s cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 549 publications below
• IF: See 148 publications below
• IP: See 1 publications below
• ELISA: See 2 publications below
• FC: See 1 publications below

Product Information

15613-1-AP targets Fibronectin in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, bovine, hamster, sheep, medaka embryos
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibronectin fusion protein Ag8004
• Full Name: fibronectin 1
• Calculated Molecular Weight: 2386 aa, 263 kDa
• Observed Molecular Weight: 250-275 kDa
• GenBank Accession Number: BC005858
• Gene Symbol: Fibronectin
• Gene ID (NCBI): 2335
• RRID: AB_2105691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibronectins play a role in cell adhesion, motility, wound healing, and the maintenance of cell shape. Fibronectins bind to cell surfaces via interactions with integrins and various molecules such as collagen, fibrin, heparin, DNA, and actin (PMID: 3326130). Cellular interaction with fibronectin (FN1) promotes cell cycle progression and proliferation by influencing cyclins. 

What is the molecular weight of FN1?

The molecular weight of FN1 is 220 kDa. 

What is the tissue specificity of FN1?

FN1 is a large glycoprotein that exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1) (PMID: 21923916). Plasma FN1, or the dimeric form, is secreted by hepatocytes, whereas cellular FN1, the dimeric or cross-linked multimeric form, is produced by fibroblasts and epithelial cells and deposited as fibrils in the extracellular matrix. 

What are the post-translational modifications of FN1?

FN1 is often sulfated and its C-terminal NC1 peptide, anastellin, is produced as a result of proteolytic processing and can inhibit tumor growth, angiogenesis, and metastasis by activating p38 MAPK and inhibiting lysophospholipid signaling (PMID: 11209058).

What is FN1's role in embryogenesis?

Fibronectin has a crucial role in the development of the left-right body axis during embryogenesis (PMID: 21466802). 

What is FN1's involvement in disease?

Fibronectin glomerulopathy is a kidney disease that eventually leads to end-stage renal disease and is caused by mutations in the FN1 gene. Mutations in FN1 lead to the production of abnormal fibronectin protein that is deposited in the glomeruli of the kidney (PMID: 18268355).

Publications

Species	Application	Title
rat		Adv Mater; Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine; Authors - Peng Lou
mouse		Cell Metab; The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity.; Authors - Suzhen Chen
mouse	IF	J Clin Invest; Mitochondrial dysfunction in macrophages promotes inflammation and suppresses repair after myocardial infarction; Authors - Shanshan Cai
human	WB	Nat Protoc; Responsive and activable nanomedicines for remodeling the tumor microenvironment.; Authors - Yinlong Zhang
human		Nat Commun; Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment; Authors - Meilin Xue
human	WB	Acta Pharm Sin B; KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer; Authors - Cancan Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Ruchi (Verified Customer) (06-02-2023)

The Ab was tried on mouse skin on diabetic wounds.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse skin

Fibronectin Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:15613-1-AP)

FH

PK (Verified Customer) (03-20-2023)

excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: fibroblast

Fibronectin Antibody Western Blot validation (1000 dilution) in fibroblast (Cat no:15613-1-AP)

FH

Susanne (Verified Customer) (08-23-2022)

under reduced conditions, blocked with 5% milk powder, incubation over night 4°C

• Applications: Western Blot
• Primary Antibody Dilution: 1 : 1000
• Cell Tissue Type: supernatant from human primary fibroblasts (serumfree !)

Fibronectin Antibody Western Blot validation (1 : 1000 dilution) in supernatant from human primary fibroblasts (serumfree !) (Cat no:15613-1-AP)

FH

Pankhuri (Verified Customer) (08-19-2022)

I tried this antibody for IF in neural stem cells. It works great in the conc. 1:200.

• Applications: Immunofluorescence
• Cell Tissue Type: Progenitor cells

Fibronectin Antibody Immunofluorescence validation ( dilution) in Progenitor cells (Cat no:15613-1-AP)

FH

Ren Jie (Verified Customer) (07-11-2022)

Very clean bands near 220 kD indicating fibronectin monomer

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human immortalized mammary fibroblasts

Fibronectin Antibody Western Blot validation (1:1000 dilution) in Human immortalized mammary fibroblasts (Cat no:15613-1-AP)

FH

SD (Verified Customer) (07-08-2022)

Good

• Applications: Western Blot
• Cell Tissue Type: Fibroblast

Fibronectin Antibody Western Blot validation ( dilution) in Fibroblast (Cat no:15613-1-AP)

FH

zunyi (Verified Customer) (03-11-2022)

This ab is used for immunohistochemistry on mouse paraffin-embedded sections. Antigen-retrieve, using pH 6.0 citric buffer, was performed prior to staining.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse bladder

FH

Lindsey (Verified Customer) (05-24-2021)

Works beautifully! Looking forward to working more with this antibody in different tissue/prep types

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human kidney (FFPE)

Fibronectin Antibody Immunohistochemistry,Immunofluorescence validation (1:100 dilution) in Human kidney (FFPE) (Cat no:15613-1-AP)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C

Fibronectin Monoclonal antibody

66042-1-Ig

Integrin Beta 1 Polyclonal antibody

12594-1-AP

Integrin alpha-5 Polyclonal antibody

10569-1-AP

CD61 / Integrin beta 3 Polyclonal antibody

18309-1-AP

Collagen Type I Polyclonal antibody

14695-1-AP

TGF Beta 1 Polyclonal antibody

18978-1-AP

Vitronectin Polyclonal antibody

15833-1-AP