Surfactant protein D Polyclonal antibody

Surfactant protein D Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11839-1-AP

Synonyms

COLEC7, Collectin 7, Lung surfactant protein D, PSP D, PSPD, SFTP4, SFTPD, SP D, surfactant protein D

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Validation Data Gallery

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  WB analysis using 11839-1-AP

  Mouse lung tissue was subjected to SDS PAGE followed by western blot with 11839-1-AP (Surfactant protein D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 11839-1-AP

  IP result of anti-Surfactant protein D (IP:11839-1-AP, 3ug; Detection:11839-1-AP 1:300) with mouse lung tissue lysate 2800ug.

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  IHC staining of mouse lung using 11839-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11839-1-AP (Surfactant protein D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 11839-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11839-1-AP (Surfactant protein D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11839-1-AP targets Surfactant protein D in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Surfactant protein D fusion protein Ag2461
• Full Name: surfactant protein D
• Calculated Molecular Weight: 375 aa, 38 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC022318
• Gene Symbol: Surfactant protein D
• Gene ID (NCBI): 6441
• RRID: AB_2254351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Surfactant protein-D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.; Authors - Guangying Yang
human	WB,IF	Hum Cell; Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells.; Authors - Hsueh-Chun Wang
rat	IHC	Transl Pediatr; Untargeted lipidomics of bronchopulmonary dysplasia induced by hyperoxia exposure in rats; Authors - Yubai Li
mouse	WB	BMC Cancer; Identification of hub genes and pathways in lung metastatic colorectal cancer; Authors - Wei Dai
mouse	WB	J Thorac Dis; Deletion of LCMR1 in alveolar type II cells induces lethal impairment of lung structure and function in adult mice; Authors - Yueming Wang
mouse	WB	Environ Pollut; LRRK2 is involved in heat exposure-induced acute lung injury and alveolar type II epithelial cell dysfunction; Authors - Yindan Wang