Surfactant protein D Polyclonal antibody

Surfactant protein D Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11839-1-AP

Synonyms

COLEC7, Collectin 7, Lung surfactant protein D, PSP D, PSPD, SFTP4, SFTPD, SP D, surfactant protein D



Tested Applications

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11839-1-AP targets Surfactant protein D in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Surfactant protein D fusion protein Ag2461
Full Name surfactant protein D
Calculated Molecular Weight 375 aa, 38 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC022318
Gene Symbol SFTPD
Gene ID (NCBI) 6441
RRIDAB_2254351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Surfactant protein-D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.

Protocols

Product Specific Protocols
WB protocol for Surfactant protein D antibody 11839-1-APDownload protocol
IHC protocol for Surfactant protein D antibody 11839-1-APDownload protocol
IP protocol for Surfactant protein D antibody 11839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.

Authors - Guangying Yang
humanWB,IF

Hum Cell

Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells.

Authors - Hsueh-Chun Wang
mouseWB

BMC Cancer

Identification of hub genes and pathways in lung metastatic colorectal cancer

Authors - Wei Dai
mouseWB

J Thorac Dis

Deletion of LCMR1 in alveolar type II cells induces lethal impairment of lung structure and function in adult mice

Authors - Yueming Wang