CD14 Polyclonal antibody

CD14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

61

Reviews (1)

Cat no : 17000-1-AP

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule

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Validation Data Gallery

• 

  WB analysis of pig spleen using 17000-1-AP

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 17000-1-AP (CD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17000-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17000-1-AP (CD14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 17000-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 17000-1-AP (CD14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 17000-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 17000-1-AP (CD14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of RAW 264.7 using 17000-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD14 antibody (17000-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HL-60 cells, human spleen tissue, RAW 264.7 cells, pig spleen tissue
• Positive IHC detected in: human tonsillitis tissue, human appendicitis tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 18 publications below
• IF: See 28 publications below
• FC: See 5 publications below

Product Information

17000-1-AP targets CD14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse, rat, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD14 fusion protein Ag10693
• Full Name: CD14 molecule
• Calculated Molecular Weight: 375 aa, 40 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC010507
• Gene Symbol: CD14
• Gene ID (NCBI): 929
• RRID: AB_2074048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Publications

Species	Application	Title
human	IF	Br J Sports Med; Chronic inflammation is a feature of Achilles tendinopathy and rupture.; Authors - Stephanie Georgina Dakin
mouse	WB	Sci Immunol; Clostridium septicum α-toxin activates the NLRP3 inflammasome by engaging GPI-anchored proteins.; Authors - Weidong Jing
human,mouse,rat	FC	Nat Commun; HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis.; Authors - Chao Liang
human	IF	EBioMedicine; Patient-derived melanoma organoid models facilitate the assessment of immunotherapies; Authors - Lingling Ou
mouse	IF	Biomaterials; Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury; Authors - Xin Liu
rat	IF	Biomaterials; Cerebro- and renoprotective activities through platelet-derived biomaterials against cerebrorenal syndrome in rat model.; Authors - Hon-Kan Yip

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue @ 1:200 with a Tris-EDTA antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Prostate

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