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Osteopontin Polyclonal antibody
Osteopontin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (1)
Applications
WB, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
197
Cat no : 22952-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, C2C12 cell, HepG2 cells, mouse kidney tissue |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 133 publications below |
| IHC | See 46 publications below |
| IF | See 46 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
22952-1-AP targets Osteopontin in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Osteopontin fusion protein Ag19216 |
| Full Name | secreted phosphoprotein 1 |
| Calculated molecular weight | 314 aa, 35 kDa |
| Observed molecular weight | 70 kDa, 44-66 kDa |
| GenBank accession number | BC007016 |
| Gene symbol | SPP1 |
| Gene ID (NCBI) | 6696 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Osteopontin antibody 22952-1-AP | Download protocol |
| IHC protocol for Osteopontin antibody 22952-1-AP | Download protocol |
| IF protocol for Osteopontin antibody 22952-1-AP | Download protocol |
| FC protocol for Osteopontin antibody 22952-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon-Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects | ||
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. | ||
Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation | ||
Nat Commun Long-lasting renewable antibacterial porous polymeric coatings enable titanium biomaterials to prevent and treat peri-implant infection. | ||
Cell Death Differ Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis | ||
J Clin Invest SIRT6 protects vascular smooth muscle cells from osteogenic transdifferentiation via Runx2 in chronic kidney disease. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Davide (Verified Customer) (09-20-2022) | The antibody works really well. Fantastic product, and the possibility to try the product with the free sample is something really appreciated. Will surely buy a full vial soon.
 |
FH WEI (Verified Customer) (03-08-2022) | Strong specific Bands
|
FH LUNFENG (Verified Customer) (01-27-2020) | VERY GOOD
|
FH Jie (Verified Customer) (01-23-2020) | Works with one specific band and one faint non-specific band. However, can be distinguished by protein size. So works fine in general.
|
FH Jingwen (Verified Customer) (01-17-2020) | This antibody showed the specific band and staining results in both western blot and immunofluorescence staining. I strongly recommend it.
|
FH Barbara (Verified Customer) (09-28-2019) | Fluorescence-based detection of OPN (green) expression in paraffin-embedded human aortic tissue, heat mediated antigen retrival with 10mM citrate buffer (pH 6,0)
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