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Osteopontin Polyclonal antibody
Osteopontin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, rat, mouse and More (1)
WB, IHC, IF, FC, CoIP, ELISA
Cat no : 22952-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, Jurkat cells, C2C12 cell, HepG2 cells, mouse kidney tissue|
|Positive IHC detected in||human stomach cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 5 publications below|
|WB||See 133 publications below|
|IHC||See 46 publications below|
|IF||See 46 publications below|
|ELISA||See 1 publications below|
|CoIP||See 1 publications below|
22952-1-AP targets Osteopontin in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, rat, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Osteopontin fusion protein Ag19216|
|Full Name||secreted phosphoprotein 1|
|Calculated molecular weight||314 aa, 35 kDa|
|Observed molecular weight||70 kDa, 44-66 kDa|
|GenBank accession number||BC007016|
|Gene ID (NCBI)||6696|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).
|Product Specific Protocols|
|WB protocol for Osteopontin antibody 22952-1-AP||Download protocol|
|IHC protocol for Osteopontin antibody 22952-1-AP||Download protocol|
|IF protocol for Osteopontin antibody 22952-1-AP||Download protocol|
|FC protocol for Osteopontin antibody 22952-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Davide (Verified Customer) (09-20-2022)
The antibody works really well. Fantastic product, and the possibility to try the product with the free sample is something really appreciated. Will surely buy a full vial soon.
WEI (Verified Customer) (03-08-2022)
Strong specific Bands
LUNFENG (Verified Customer) (01-27-2020)
Jie (Verified Customer) (01-23-2020)
Works with one specific band and one faint non-specific band. However, can be distinguished by protein size. So works fine in general.
Jingwen (Verified Customer) (01-17-2020)
This antibody showed the specific band and staining results in both western blot and immunofluorescence staining. I strongly recommend it.
Barbara (Verified Customer) (09-28-2019)
Fluorescence-based detection of OPN (green) expression in paraffin-embedded human aortic tissue, heat mediated antigen retrival with 10mM citrate buffer (pH 6,0)