MCL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MCL1 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

116

Rezensionen (1)

Kat-Nr. : 16225-1-AP

Synonyme

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

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Galerie der Validierungsdaten

• 

  WB analysis using 16225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16225-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 16225-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16225-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16225-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16225-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 16225-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 16225-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 16225-1-AP

  IP Result of anti-MCL1 (IP:16225-1-AP, 3ug; Detection:16225-1-AP 1:1000) with Raji cells lysate 800ug.

• 

  IF Staining of MCF-7 using 16225-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MCL1 antibody (16225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Ramos using 16225-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.5 ug Anti-Human MCL1 (16225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen, HEK-293T-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Hirngewebe, humanes Lebergewebe, K-562-Zellen, Mausmilzgewebe, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 100 publications below
• IHC: See 13 publications below
• IF: See 5 publications below
• IP: See 5 publications below

Produktinformation

16225-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MCL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCL1 fusion protein Ag10397
• Vollständiger Name: myeloid cell leukemia sequence 1 (BCL2-related)
• Berechnetes Molekulargewicht: 350 aa, 37 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC107735
• Gene symbol: MCL1
• Gene ID (NCBI): 4170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)

Publikationen

Species	Application	Title
mouse	IHC	Nat Commun; MCL-1 gains occur with high frequency in lung adenocarcinoma and can be targeted therapeutically.; Authors - Enkhtsetseg Munkhbaatar
human	WB	Nat Commun; Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.; Authors - Di Hu
mouse	WB	Nat Commun; IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation.; Authors - Senthil Kumaran Satyanarayanan
human	WB	EBioMedicine; The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.; Authors - Pingbo Chen
human	WB,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
human	WB	Aging (Albany NY); Melatonin induces cell cycle arrest and suppresses tumor invasion in urinary bladder urothelial carcinoma; Authors - Tzuo-Yi Hsieh

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Eya (Verified Customer) (07-06-2021)

the bands appear very clear

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: shTHP1

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