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Bcl-XL Polyclonal antibody

Bcl-XL Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10783-1-AP

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL



Tested Applications

Positive WB detected inA549 cells, Raji cells, Jurkat cells, rat brain tissue, HeLa cells, NIH/3T3 cells, human placenta tissue, C6 cells, K-562 cells, mouse placenta tissue, RAW264.7
Positive IP detected ink-562 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10783-1-AP targets Bcl-XL in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, sheep, zebrafish, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag1232
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
RRIDAB_2064724
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta) , and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).

Protocols

Product Specific Protocols
WB protocol for Bcl-XL antibody 10783-1-APDownload protocol
IHC protocol for Bcl-XL antibody 10783-1-APDownload protocol
IF protocol for Bcl-XL antibody 10783-1-APDownload protocol
IP protocol for Bcl-XL antibody 10783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Immunity

cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity

Authors - Yunlong Zhao
humanWB

Nat Commun

Structural and functional analyses of hepatitis B virus X protein BH3-like domain and Bcl-xL interaction.

Authors - Tian-Ying Zhang
humanWB

Carbohydr Polym

A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer

Authors - Shaofeng Duan
human,mouseWB

Cancer Res

Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.

Authors - Yogev Sela
ratWB

Oxid Med Cell Longev

Cyclic Polypeptide D7 Protects Bone Marrow Mesenchymal Cells and Promotes Chondrogenesis during Osteonecrosis of the Femoral Head via Growth Differentiation Factor 15-Mediated Redox Signaling.

Authors - Jiazheng Chen
ratWB

Oxid Med Cell Longev

Smad7 Is Highly Expressed in Human Degenerative Discs and Participates in IL-1β-Induced Apoptosis of Rat AF Cells via the Mitochondria Pathway.

Authors - Bo Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (10-26-2023)

In cancer cell lines, the bands appeared significantly clearer, and the optimal concentration was found to be 1:1000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Lung cancer cell lines
Bcl-XL Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines (Cat no:10783-1-AP)
FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with PiP5Ka antibody (15713-1-AP) during 50min After, a seconde staining was performed with 0,05µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (10783-1-AP) +secondary antibody

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 0,05µl/10^6 cells
  • Cell Tissue Type: B cells
Bcl-XL Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:10783-1-AP)