BCL2L1 Polyclonal antibody

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BCL2L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10783-1-AP Copied!

Publications (252)

Reviews (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

Bcl-xL, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1, Bcl-2-like protein 1

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  WB analysis using 10783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HeLa cells using 10783-1-AP

  WB result of Bcl-XL antibody (10783-1-AP, 1:800) with si-Control and si-Bcl-XL transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Caco-2 using 10783-1-AP

  WB result of BCL2L1 antibody (10783-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL2L1 transfected Caco-2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of C6 using 10783-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 10783-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 10783-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 10783-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse placenta using 10783-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of RAW264.7 using 10783-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 10783-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 10783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 10783-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 10783-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10783-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of k-562 using 10783-1-AP

  IP result of anti-Bcl-XL (IP:10783-1-AP, 4ug; Detection:10783-1-AP 1:1000) with k-562 cells lysate 1760 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 10783-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10783-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 10783-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10783-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 10783-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10783-1-AP (Bcl-XL antibody at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 10783-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10783-1-AP (Bcl-XL antibody at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 10783-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl-XL antibody (10783-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, C6 cells, Caco-2 cells, HeLa cells, human placenta tissue, Jurkat cells, K-562 cells, mouse placenta tissue, NIH/3T3 cells, Raji cells, rat brain tissue, RAW264.7
• Positive IP detected in: k-562 cells
• Positive IHC detected in: human lung cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10783-1-AP targets BCL2L1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, zebrafish, hamster, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1232

  Product name: Recombinant human Bcl-xL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-233 aa of BC019307

  Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK

• Full Name: BCL2-like 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC019307
• Gene Symbol: BCL2L1
• Gene ID (NCBI): 598
• RRID: AB_2064724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q07817
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK, or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).

Publications

What published studies show

Anti-BCL2L1 (Bcl-xL), Cat# 10783-1-AP has 252 citations. Publications appear in high-impact journals including Immunity, Nature Communications, Cancer Research, Cell Death & Differentiation, Biomaterials, Oncogene, Free Radical Biology and Medicine, British Journal of Cancer, Oxidative Medicine and Cellular Longevity, International Journal of Biological Sciences, Phytomedicine, Science Reports, and Molecular Therapy. Research fields span apoptosis, cancer biology, ferroptosis, mitochondrial regulation, drug discovery, neuroprotection, liver disease, inflammation, and autophagy.

Automatically generated from the 252 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Immunity; cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity; Authors - Yunlong Zhao
mouse,human	WB	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
human	WB	Nat Commun; Structural and functional analyses of hepatitis B virus X protein BH3-like domain and Bcl-xL interaction.; Authors - Tian-Ying Zhang
human	WB	J Nanobiotechnology; ROS-responsive EGCG-Fe-Cur nanosystems for radioprotection through antioxidant and inhibition of ferroptosis pathways.; Authors - Xinrui Yang
human	WB	Acta Pharm Sin B; Avenanthramide A potentiates Bim-mediated antineoplastic properties of 5-fluorouracil via targeting KDM4C/MIR17HG/GSK-3β negative feedback loop in colorectal cancer; Authors - Rong Fu
human	WB	J Exp Clin Cancer Res; ONC213: a novel strategy to resensitize resistant AML cells to venetoclax through induction of mitochondrial stress; Authors - Jenna L Carter

Reviews

4.2 out of 5

5 verified customer reviews

What customers say

Users generally rate this BCL-XL antibody positively for Western blot, with clear bands at dilutions of 1:800–1:2000. One reviewer noted a few nonspecific bands above 37 kD when detecting endogenous BCL2L1. For cancer cell lines, the optimal dilution was found to be 1:1000 with significantly clearer results. The antibody has also been successfully used for immunohistochemistry, immunofluorescence, and flow cytometry (intracellular staining of mouse B cells). Overall, users find it easy to work with and reliable for detecting BCL-XL across multiple applications.

Automatically generated from the 5 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Mounika (Verified Customer) (03-24-2026)

very easy antibody to work with and played important role in research project!

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:800

Sai Sindhura (Verified Customer) (01-08-2026)

BCL-XL gives very good bands

• Applications: Western Blot, Other
• Primary Antibody Dilution: 1:1000

N (Verified Customer) (11-04-2025)

This Ab is okay to detect endo BCL2L1 (30 kD), although there are a few nonspecific bands above 37 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: PC12 cell lysate

Shinford (Verified Customer) (10-26-2023)

In cancer cell lines, the bands appeared significantly clearer, and the optimal concentration was found to be 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lung cancer cell lines

BCL2L1 Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines (Cat no:10783-1-AP)

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with PiP5Ka antibody (15713-1-AP) during 50min After, a seconde staining was performed with 0,05µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (10783-1-AP) +secondary antibody

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0,05µl/10^6 cells
• Cell Tissue Type: B cells

BCL2L1 Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:10783-1-AP)