- Featured Product
- KD/KO Validated
AKT Polyclonal antibody
AKT Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10176-2-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse liver tissue, rat brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human ovary tumor tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, mouse brain tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10176-2-AP targets AKT in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, goat, chicken, zebra finches, fish, Zebrafish, hamster, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AKT fusion protein Ag0213|
|Full Name||v-akt murine thymoma viral oncogene homolog 1|
|Calculated molecular weight||56 kDa|
|Observed molecular weight||56-62 kDa|
|GenBank accession number||BC000479|
|Gene ID (NCBI)||207|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
|Product Specific Protocols|
|WB protocol for AKT antibody 10176-2-AP||Download protocol|
|IHC protocol for AKT antibody 10176-2-AP||Download protocol|
|IF protocol for AKT antibody 10176-2-AP||Download protocol|
|IP protocol for AKT antibody 10176-2-AP||Download protocol|
|FC protocol for AKT antibody 10176-2-AP||Download protocol|
|Click here to view our Standard Protocols|
RAB31 marks and controls an ESCRT-independent exosome pathway.
Nat Cell Biol
Heat stress activates YAP/TAZ to induce the heat shock transcriptome.
Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk
Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.
Acta Pharm Sin B
Oral nano-formulation improves pancreatic islets dysfunction via lymphatic transport for antidiabetic treatment
MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hyopil (Verified Customer) (08-23-2021)
Worked well with western blotting
Azita (Verified Customer) (06-02-2021)
Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.