AKT Polyclonal antibody

• Featured Product
• KD/KO Validated

AKT Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, FC, CoIP, , ELISA

Conjugate

Unconjugated

Publications

717

Reviews (2)

Cat no : 10176-2-AP

Synonyms

AKT, AKT1, AKT2, AKT3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 10176-2-AP

  WB result of AKT antibody (10176-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected MCF-7 cells.

• 

  WB analysis of HeLa cells using 10176-2-AP

  siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10176-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10176-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10176-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:500.

• 

  IP experiment of HeLa using 10176-2-AP

  IP Result of anti-AKT1 (IP:10176-2-AP, 3ug; Detection:10176-2-AP 1:500) with HeLa cells lysate 2500ug.

• 

  IHC staining of human ovary tumor using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10176-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10176-2-AP (AKT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10176-2-AP

  1X10^6 HeLa cells were stained with 0.2ug AKT1 antibody (10176-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse liver tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 697 publications below
• IHC: See 18 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

10176-2-AP targets AKT in WB, IP, IHC, IF, FC, CoIP, , ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, chicken, fish, goat, hamster, Megalobrama amblycephala, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKT fusion protein Ag0213
• Full Name: v-akt murine thymoma viral oncogene homolog 1
• Calculated molecular weight: 56 kDa
• Observed molecular weight: 56-62 kDa
• GenBank accession number: BC000479
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Publications

Species	Application	Title
human	WB	Med Sci Monit; PMID: 30605443; Authors - Lin Chen
rat	WB	Asian J Androl; Human tissue kallikrein-1 protects against the development of erectile dysfunction in a rat model of hyperhomocysteinemia.; Authors - Kai Cui
Megalobrama amblycephala	WB	Fish Physiol Biochem; Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala.; Authors - Hualiang Liang
human	WB	Biochem Biophys Res Commun; Long non-coding RNA FAM84B-AS promotes resistance of gastric cancer to platinum drugs through inhibition of FAM84B expression.; Authors - Yeqian Zhang
human	WB	J Biomed Nanotechnol; Nano-Gold Loaded with Resveratrol Enhance the Anti-Hepatoma Effect of Resveratrol In Vitro and In Vivo.; Authors - Duoduo Zhang
human	WB	Inflammation; Umbilical Cord Blood Mesenchymal Stem Cells Enhance Lipopolysaccharide-Induced IL-10 and IL-37 Production in THP-1 Cells.; Authors - Ting Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hyopil (Verified Customer) (08-23-2021)

Worked well with western blotting

• Applications: Western Blot
• Cell Tissue Type: HAP1

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: NSC34

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