CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, plant, Zebrafish

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

7D2C10

Cat no : CL488-60008

Synonyms

ACTB, Actin, beta Actin

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Validation Data Gallery

• 

  WB analysis using CL488-60008

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-60008 (Beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60008 targets Beta Actin in WB applications and shows reactivity with human, mouse, rat, plant, Zebrafish samples.

• Tested Reactivity: human, mouse, rat, plant, Zebrafish
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: Beta Actin fusion protein Ag0297
• Full Name: actin, beta
• Calculated Molecular Weight: 375 aa, 42 kDa
• GenBank Accession Number: BC002409
• Gene Symbol: Beta Actin
• Gene ID (NCBI): 60
• RRID: AB_2883089
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm), and can recognize all the actins.