Various lysates were subjected to SDS PAGE followed by western blot with CL594-60008 (Beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with CL594-60008 (Beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using CL594-60008
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.
IF Staining of MDCK using CL594-60008
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions
Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Background Information
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody is conjugated with CL594,Ex/Em 593 nm/614 nm.
Protocols
Product Specific Protocols
WB protocol for CL594 Beta Actin antibody CL594-60008
Various lysates were subjected to SDS PAGE followed by western blot with CL594-60008 (Beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF/ICC Figures
IF Staining of HeLa using CL594-60008
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.
IF Staining of MDCK using CL594-60008
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Beta Actin Monoclonal antibody
Catalog Number
CL594-60008
Citations
4
Dilutions
WB : 1:1000-1:4000 IF/ICC : 1:200-1:1000
Applications
WB, IF/ICC
Reactivity
human, mouse, rat, plant, zebrafish, canine
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
