HRP-conjugated Beta Actin Monoclonal antibody
Beta Actin Monoclonal Antibody for WB
Host / Isotype
Mouse / IgM
Reactivity
human, mouse, rat, zebrafish, plant and More (5)
Applications
WB, IF, ELISA
Conjugate
HRP
374
CloneNo.
7D2C10
Cat no : HRP-60008
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, multi-cells/tissue, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 361 publications below |
| IF | See 2 publications below |
| ELISA | See 1 publications below |
Product Information
HRP-60008 targets Beta Actin in WB, IF, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.
| Tested Reactivity | human, mouse, rat, zebrafish, plant |
| Cited Reactivity | human, mouse, rat, pig, canine, monkey, chicken, hamster |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant protein |
| Full Name | actin, beta |
| Calculated Molecular Weight | 375 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC002409 |
| Gene Symbol | Beta Actin |
| Gene ID (NCBI) | 60 |
| RRID | AB_2819183 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP Beta Actin antibody HRP-60008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate | ||
Gastroenterology Dual inhibition of CDK4/6 and XPO1 induces senescence with acquired vulnerability to CRBN-based PROTAC drugs | ||
J Hematol Oncol METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation | ||
Nat Neurosci Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sara (Verified Customer) (10-05-2024) | I love Proteintech HRP-conjugated antibodies. These are def my favourites for WB controls (housekeeping proteins), I have got actin, tubulin and GAPDH. Very good bands, super reliable.
|
FH Maria (Verified Customer) (10-24-2023) | Used Incubation over night at 4°C, never tested a shorter incubation, but it worked very nice.
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FH Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells andmice tissue
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FH Wojciech (Verified Customer) (04-20-2023) | The beta actin works very well diluted at 1:5000. It was used on T-ALL cell lines. Loading 30 ug of whole protein lysate.
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FH Priya (Verified Customer) (04-17-2023) | Used for Caco2 cells
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FH Iru (Verified Customer) (06-07-2021) | The antibody works well.
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FH Azita (Verified Customer) (06-02-2021) | Western blot analysis using HRP- conjugated beta actin in NSC34 cell line at dilution of 1:10000
 |
FH Hasibur (Verified Customer) (08-01-2019) | Nice antibody and no need for secondary antibody
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FH Joanne (Verified Customer) (01-22-2019) | Tested using 10ug of Caco-2 cell lysate and whole membrane. A strong band with no background and a great time saver!
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FH Tess (Verified Customer) (11-29-2018) | Little to no background, and takes half the time.
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