Biotin-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, pig, plant, Zebrafish

Applications

WB, IHC

Conjugate

Biotin

Publications

4

CloneNo.

7D2C10

Cat no : Biotin-60008

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, PS1TP5BP1, β actin

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Validation Data Gallery

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  IHC staining of mouse heart using Biotin-60008

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-60008 (beta Actin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using Biotin-60008

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-60008 (beta Actin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

Biotin-60008 targets Beta Actin in WB, IHC applications and shows reactivity with human, mouse, rat, pig, plant, Zebrafish samples.

• Tested Reactivity: human, mouse, rat, pig, plant, Zebrafish
• Cited Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: Beta Actin fusion protein Ag0297
• Full Name: actin, beta
• Calculated Molecular Weight: 375 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC002409
• Gene Symbol: Beta Actin
• Gene ID (NCBI): 60
• RRID: AB_2883062
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Publications

Species	Application	Title
human	WB	iScience; The vesicular transporter STX11 governs ATGL-mediated hepatic lipolysis and lipophagy.; Authors - Gaojian Zhang
pig	WB	Reproduction; FSH promotes immature porcine Sertoli cell proliferation by activating the CCR7/Ras-ERK signaling axis; Authors - Yanfei Yin
rat	WB	Exp Ther Med; Deciphering the role of TNF‑α‑induced protein 8‑like 2 in the pathogenesis of necrotizing enterocolitis in neonatal rats; Authors - Juan Luo
human	WB	Balkan Med J; Asiaticoside Down-Regulates HIF-1α to Inhibit Proliferation, Migration, and Angiogenesis in Thyroid Cancer Cells; Authors - Yi Zhang