ChromoTek RFP-Trap Magnetic Agarose, kit

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

Specificity

mCherry, mPlum, mRFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Cat no : rtmak

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.

DescriptionThe RFP-Trap® Magnetic Agarose Kit contains RFP-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of RFP-fusion proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity22.5 μg of recombinant RFP per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 5 nM
Compatibility with mass spectrometryThe RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631363
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
RFP-Trap® Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
IP

Commun Biol

βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity.

Authors - Sayan Ghosh
IP

Commun Biol

βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells.

Authors - Peng Shang
IP

mBio

CDC50 Orthologues in Plasmodium falciparum Have Distinct Roles in Merozoite Egress and Trophozoite Maturation.

Authors - Avnish Patel
IP

Cell Rep

RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss.

Authors - Stylianos Kosmidis

Sci Adv

Transient RNA structures cause aberrant influenza virus replication and innate immune activation

Authors - Hollie French

Biochimie

Irradiation potentiates p53 phosphorylation and p53 binding to the promoter and coding region of the TP53 gene

Authors - Soňa Legartová