ChromoTek RFP-Trap® Magnetic Particles M-270

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

mCherry, mPlum, mRFP

Applications

IP, CoIP

Conjugate

Magnetic Particles M-270

Type

Nanobody

Cat no : rtd

Synonyms

Magnetic Particles M-270, RFP Magnetic Particles M-270, RFP tag, RFP-tag, RFP-Trap® , RFP-Trap® Magnetic Particles M-270, rtd, rtd-10, rtd-100, rtd-20, rtd-200, rtd-400



Product Information

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

DescriptionImmunoprecipitation of RFP-fusion proteins and their interacting factors with anti-RFP Nanobody conjugated to magnetic particles. RFP-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP.

• IP and Co-IP of RFP-tagged proteins without size limitation

• Automation and high throughput applications

• Magnetic separation with easy and efficient washing of RFP-Trap Magnetic Particles M-270

• Very high affinity (KD=5 nM) to bind even low abundant proteins

• No heavy & light antibody chains, short incubation (5-30 min)

• Extraordinary binding, also under harsh conditions

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity1.25 μg of recombinant mCherry per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility0.5 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 5 nM
Compatibility with mass spectrometryThe RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2861253
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
ChIP

Plant Cell

Diversification of heat shock transcription factors expanded thermal stress responses during early plant evolution.

Authors - Ting-Ying Wu
IP

Cell Rep

RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site.

Authors - Jasmine George
WB

New Phytol

Increasing amyloplast size in wheat endosperm through mutation of PARC6 affects starch granule morphology

Authors - Lara Esch
CoIP

Mol Biol Cell

FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans

Authors - Sterling C T Martin