ChromoTek iST RFP-Trap® Kit for AP-MS sample preparation of RFP-fusion proteins

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

Specificity

mCherry, mPlum, mRFP

Applications

IP, MS

Type

Nanobody

Conjugate

Agarose

Cat no : rtak-iST

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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  Schematic workflow of iST Nano-Trap® Kit

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  Save up to 40 hours on your mass spec experiments with the iST Nano-Trap® Kits

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Product Information

Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)

• Description: The iST RFP-Trap® Kit for processing RFP-fusion proteins and their interacting partners for mass spectrometry analysis: The kit includes the RFP-Trap for IP/Co-IP of RFP-fusion proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:

  • Reliable dentification of RFP-fusion proteins & interacting partners by MS

  • Identification of even low abundant RFP-fusion proteins

  • Superior processing of IP samples into clean peptide mixtures

  • Intuitive protocol for reproducible results

• Applications: IP, MS
• Specificity/Target: mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 22.5 μg of recombinant RFP per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 5 nM
• Compatibility with mass spectrometry: The RFP-Trap® is optimized for on-bead digestion.
• Storage Buffer: 20% ethanol
• Storage Condition: Kit is shipped at ambient temperature. Upon receipt store RFP-Trap at 4°C and Digest at -20°C

Kit components

Component	Description
RFP-Trap Agarose	8 reactions plus 2 controls
Digest	Enzyme Trypsin-mix to digest proteins.
Resuspend buffer	Protease reconstitution buffer for enzymes.
Lyse buffer	Denature, reduce and alkylate proteins.
Stop solution	Stop the enzymatic activity.
Wash 1 buffer	Clean up peptides from hydrophobic contaminants.
Wash 2 buffer	Clean up peptides from hydrophilic contaminants.
Elution buffer	Elute the peptides from the cartridge.
LC-Load load	Load peptides on reversed-phase LC-MS column.
Cartridges	Cartridge for 1 to 100 µg protein starting material.
Waste tubes	Tube for collecting waste after washing steps.
Collection tubes	Tube for collecting peptides after elution.
Adapters	Enables placing a cartridge into a tube.
Caps	Cap to optionally close the cartridge’s bottom.