Cat no : rtak
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
|Description||The RFP-Trap® Agarose Kit contains RFP-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of RFP-fusion proteins and their interacting factors.
• Fast, reliable & efficient one-step immunoprecipitation
• No heavy & light antibody chains on the SDS-PAGE
• Stable under harsh washing conditions
• Short incubation time (30 min)
|Applications||IP, CoIP, ChIP, RIP|
|Specificity/Target||mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
For the complete list, please click here: Fluorescent protein specificity table
|Binding capacity||22.5 μg of recombinant RFP per 25 μL bead slurry|
|Conjugate||Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)|
|Elution buffer||SDS sample buffer|
0.2 M glycine pH 2.5
|Wash buffer compatibility||10 mM DTT, 4 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % Triton X-100|
|Affinity (KD)||Dissociation constant KD of 5 nM|
|Compatibility with mass spectrometry||The RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!|
|RFP-Trap® Agarose||20 reactions (500 µL)|
|Lysis buffer||Optimized for cytoplasmatic proteins and mammalian cell lysis|
|RIPA buffer||Optimized for nuclear/chromatin proteins and mammalian cell lysis|
|Wash buffer||Removal of unwanted proteins, peptides, etc.|
|Dilution buffer||Dilution of cell lysate|
|Elution buffer||For acidic elution|
|SDS RFP-Trap Agarose Kit (PDF)|
|Protocol RFP-Trap Agarose Kit (PDF)|
|Split Fluorescent Protein Technology (PDF)|
|Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins|
|Product brochure (PDF)|
|Fluorescent protein specificity table (PDF)|
|Troubleshooting guide immunoprecipitation (IP) (PDF)|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ziqiao (Verified Customer) (06-10-2022)
The eluted proteins were much purified but only weaknesses is that the amount was very limited. The acid elution method might be optimized.