PE-conjugated MYC tag Monoclonal antibody

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  FC experiment of Transfected HEK-293 using PE-60003-2

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.2 ug PE N/a MYC Tag Monoclonal Antibody (PE-60003-2, Clone:1A5A2), and 0.2 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MYC tag Monoclonal Antibody for FC (Intra)

Cat No. PE-60003-2

Clone No.1A5A2

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

FC (Intra)

Synonyms

myc, EQKLISEEDL, MYC-tag

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Tested Applications

• Positive FC (Intra) detected in: Transfected HEK-293 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-60003-2 targets MYC tag in FC (Intra) applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Myc tag
• Gene Symbol: Myc tag
• Gene ID (NCBI): 99
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.