CoraLite® Plus 647-conjugated MYC tag Monoclonal antibody

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  IF Staining of Transfected HEK-293 using CL647-60003

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647-conjugated MYC tag antibody (CL647-60003, Clone: 1A5A2 ) at dilution of 1:800.

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  FC experiment of HEK-293 transfected with GFP-MYC using CL647-60003

  1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated MYC tag Monoclonal antibody (CL647-60003, Clone:1A5A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293 using CL647-60003

  1X10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-MYC tag (CL647-60003, Clone:1A5A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MYC tag Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL647-60003

Clone No.1A5A2

Publications(1)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

IF/ICC, FC (Intra)

Synonyms

EQKLISEEDL

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Tested Applications

• Positive IF/ICC detected in: Transfected HEK-293 cells
• Positive FC (Intra) detected in: Transfected HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60003 targets MYC tag in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Myc tag
• Gene Symbol: Myc tag
• Gene ID (NCBI): 99
• RRID: AB_2920241
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Publications

Species	Application	Title
		Biochem Pharmacol; Peptide-based CAR-NK cells: A novel strategy for the treatment of solid tumors; Authors - Qianqian Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lisbeth (Verified Customer) (10-20-2023)

Used for detecting myc-tag labelled proteins on cell surfaces, even after fixation.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:50