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CoraLite® Plus 647-conjugated MYC tag Monoclonal antibody

MYC tag Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1A5A2

Cat no : CL647-60003

Synonyms

EQKLISEEDL

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using CoraLite® Plus 647-conjugated MYC tag Monoclonal a (CL647-60003)

    IF Staining of Transfected HEK-293 using CL647-60003

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647-conjugated MYC tag antibody (CL647-60003, Clone: 1A5A2 ) at dilution of 1:800.

  • Flow cytometry (FC) experiment of Transfected HEK-293 cells using CoraLite® Plus 647-conjugated MYC tag Monoclonal a (CL647-60003)

    FC experiment of Transfected HEK-293 using CL647-60003

    1X10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-MYC tag (CL647-60003, Clone:1A5A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60003 targets MYC tag in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name MYC tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2920241
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 MYC tag antibody CL647-60003Download protocol
FC protocol for CL Plus 647 MYC tag antibody CL647-60003Download protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Lisbeth (Verified Customer) (10-20-2023)

Used for detecting myc-tag labelled proteins on cell surfaces, even after fixation.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 1:50