MYC tag Monoclonal antibody
MYC tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (1)
Applications
WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
245
CloneNo.
1A5A2
Cat no : 60003-2-Ig
Synonyms
"MYC tag Antibodies" Comparison
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Tested Applications
| Positive WB detected in | recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 175 publications below |
| IF | See 21 publications below |
| IP | See 58 publications below |
| CoIP | See 33 publications below |
| ChIP | See 1 publications below |
Product Information
60003-2-Ig targets MYC tag in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | MYC tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2734122 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| IP protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| FC protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Discov Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability | ||
Nat Cancer Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism | ||
Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence | ||
Nat Commun UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean | ||
Am J Respir Crit Care Med Loss of DP1 Aggravates Vascular Remodeling in Pulmonary Arterial Hypertension via mTORC1 Signaling. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH RASHMI (Verified Customer) (07-26-2024) | Used this product, highly recommended
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FH Sarah (Verified Customer) (10-27-2023) | works well at low dilution for WB and worked for immunoprecipitation
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FH manohar (Verified Customer) (07-10-2023) | Used Nitrocellulose membrane with 5% milk as blocking, antibody diluted in 1% milk.
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FH Amy (Verified Customer) (03-05-2023) | Clean bands for detecting over expressed Myc-tagged proteins in HEK293T cell lysates.
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FH Isabelle Cristine (Verified Customer) (01-14-2022) | The antibody works generally well for the detection of MYC-tagged proteins.
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FH Tom (Verified Customer) (02-10-2021) | HEK293T cells were transfected with a Myc-tagged construct. The 60003-2-Ig antibody (1:2000) resulted in a lot of background. An alternative antibody was used alongside this one, with a stronger band and less background being achieved.
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FH Alinda (Verified Customer) (10-09-2019) | This antibody was used at 1:1000 in HEK cells transfected with a myc-tagged insert cloned into an overxpression. Cells were blocked for 1 hr at room temp in 5%goat serum PBST.This antibody has a lot of background; another antibody from cell signalling was tested in parallel and performed much better
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