HRP-conjugated MYC tag Monoclonal antibody

MYC tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB

Conjugate

HRP

CloneNo.

1A5A2

Cat no : HRP-60003

Synonyms

myc, MYC-tag, EQKLISEEDL

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Validation Data Gallery

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  WB analysis using HRP-60003

  Various lysates were subjected to SDS PAGE followed by western blot with HRP-60003 (MYC tag antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

HRP-60003 targets MYC tag in WB applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: MYC tag
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.