CoraLite® Plus 750-conjugated MYC tag Monoclonal antibody

MYC tag Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IF/ICC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1A5A2

Cat no : CL750-60003

Synonyms

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Validation Data Gallery

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  WB analysis using CL750-60003

  HEK-293 cell lysates and Recombinant protein(Ag19172) were subjected to SDS PAGE followed by western blot with CL750-60003 (MYC tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected HEK-293 using CL750-60003

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 750 MYC tag antibody (CL750-60003, Clone: 1A5A2 ) at dilution of 1:100.

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IF/ICC detected in: Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-60003 targets MYC tag in WB, IF/ICC applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: MYC tag
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.