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Lamin B1 Monoclonal antibody
Lamin B1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 66095-1-Ig
"Lamin B1 Antibodies" Comparison
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|Positive WB detected in||multi-cells/tissue, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human pancreas cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, mouse eye tissue, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:10000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66095-1-Ig is Lamin B1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Rabbit, zebrafish|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Lamin B1 fusion protein Ag20522|
|Full Name||lamin B1|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||66-70 kDa|
|GenBank accession number||BC012295|
|Gene ID (NCBI)||4001|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.
|Product Specific Protocols|
|IHC protocol for Lamin B1 antibody 66095-1-Ig||Download protocol|
|IF protocol for Lamin B1 antibody 66095-1-Ig||Download protocol|
|IP protocol for Lamin B1 antibody 66095-1-Ig||Download protocol|
|FC protocol for Lamin B1 antibody 66095-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Nat Cell Biol
Ceramide-rich microdomains facilitate nuclear envelope budding for non-conventional exosome formation.
OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.
Cell Host Microbe
Structural Basis for a Species-Specific Determinant of an SIV Vif Protein toward Hominid APOBEC3G Antagonism.
Cell Host Microbe
The Nuclear Matrix Protein SAFA Surveils Viral RNA and Facilitates Immunity by Activating Antiviral Enhancers and Super-enhancers.
Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.
Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (03-08-2022)
Specfic bands around predicted size with higher non-specific bands
P. (Verified Customer) (05-15-2021)
Eiko (Verified Customer) (11-18-2020)
Used RIPA buffer for western sample preparation, and 5% Skim milk TBS-T was used for blocking and antibody dilution.Since I could see clear lamin B1 signal, I am happy to use this antibody as one of my internal controls.
Tom (Verified Customer) (10-09-2020)
I observed a discrete ~70kDa Lamin B1 band using this antibody.
Yuan (Verified Customer) (12-13-2019)
Did staining for human A549 cell.The Lamin B antibody had high background in nucleus. Please see attached image.
Shubham (Verified Customer) (03-14-2019)