CoraLite®555-conjugated Lamin B1 Monoclonal antibody

• Featured Product
• KD/KO Validated

Lamin B1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3C10G12

Cat no : CL555-66095

Synonyms

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

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Validation Data Gallery

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  IF Staining of U2OS using CL555-66095

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite®555 Lamin B1 antibody (CL555-66095, Clone: 3C10G12 ) at dilution of 1:1000.

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  IF Staining of HepG2 using CL555-66095

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 Lamin B1 antibody (CL555-66095, Clone: 3C10G12 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-66095

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Lamin B1 (CL555-66095, Clone:3C10G12) (red), or 0.4 ug isotype control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66095 targets Lamin B1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Lamin B1 fusion protein Ag20522
• Full Name: lamin B1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC012295
• Gene Symbol: Lamin B1
• Gene ID (NCBI): 4001
• RRID: AB_2919674
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.