Lamin B1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Lamin B1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

3C10G12

Cat no : 66095-1-PBS

Synonyms

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

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Validation Data Gallery

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  WB analysis using 66095-1-Ig (same clone as 66095-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66095-1-Ig (Lamin B1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  WB analysis of multi-cells/tissue using 66095-1-Ig (same clone as 66095-1-PBS)

  Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-Ig) at dilution 1:20000. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

  WB result of Lamin B1 antibody (66095-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

  IP result of anti-Lamin B1 (IP:66095-1-Ig, 4ug; Detection:66095-1-Ig 1:20000) with HeLa cells lysate 3560ug. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66095-1-Ig (same clone as 66095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66095-1-Ig (same clone as 66095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66095-1-Ig (same clone as 66095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 66095-1-Ig (same clone as 66095-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Lamin B1 antibody (66095-1-Ig, Clone: 3C10G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66095-1-Ig (same clone as 66095-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:500 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (66095-1-Ig, Clone:3C10G12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

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Product Information

66095-1-PBS targets Lamin B1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Lamin B1 fusion protein Ag20522
• Full Name: lamin B1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC012295
• Gene Symbol: Lamin B1
• Gene ID (NCBI): 4001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.