1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Mouse IgG1 Isotype Control (65124-1-Ig, Clone:MOPC-21) (red) or 0.2ug Anti-Human CD8a (65113-1-Ig, Clone:Hit8a) (green) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000 or unstained (blue). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Mouse IgG1 Isotype Control (65124-1-Ig, Clone:MOPC-21) (red) or 0.2ug Anti-Human CD8a (65113-1-Ig, Clone:Hit8a) (green) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000 or unstained (blue). Cells were not fixed. Lymphocytes were gated.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65124-1-Ig targets IgG1 Isotype Control in FC applications and shows reactivity with n/a samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
Product Specific Protocols
FC protocol for IgG1 Isotype Control antibody 65124-1-Ig
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Mouse IgG1 Isotype Control (65124-1-Ig, Clone:MOPC-21) (red) or 0.2ug Anti-Human CD8a (65113-1-Ig, Clone:Hit8a) (green) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000 or unstained (blue). Cells were not fixed. Lymphocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG1 Isotype Control Monoclonal antibody
Catalog Number
65124-1-Ig
Citations
-
Dilutions
Applications
FC
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
