CoraLite®594-conjugated Lamin B1 Monoclonal antibody

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• KD/KO Validated

Lamin B1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

3

CloneNo.

3C10G12

Cat no : CL594-66095

Synonyms

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66095

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Lamin B1 antibody (CL594-66095, Clone: 3C10G12 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66095

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Lamin B1 (CL594-66095, Clone:3C10G12) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 3 publications below

Product Information

CL594-66095 targets Lamin B1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Lamin B1 fusion protein Ag20522
• Full Name: lamin B1
• Calculated Molecular Weight: 66 kDa
• GenBank Accession Number: BC012295
• Gene Symbol: Lamin B1
• Gene ID (NCBI): 4001
• RRID: AB_2883510
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publications

Species	Application	Title
human	IF	Redox Biol; MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway.; Authors - Shanshan Ma
human	IF	Mol Oncol; Small extracellular vesicles deliver TGF-β1 and promote adriamycin resistance in breast cancer cells.; Authors - Chunli Tan
human	IF	bioRxiv; Replicative senescence is ATM driven, reversible, and accelerated by hyperactivation of ATM at normoxia; Authors - Alexander Stuart

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