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Foxp3 / Transcription Factor Staining Buffer Kit

Application:

FC

Cat no : PF00011

Validation Data Gallery

  • 1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).

    1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).

Product Information

The Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. Included are the following components, to be used during staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma.

   Components

Size

 Foxp3 / Transcription Factor Fix/Perm Concentrate (4X)   PF00011-A

50 mL

Foxp3 / Transcription Factor Fix/Perm Diluent (1X)   PF00011-B

150 mL

Flow Cytometry Perm Buffer (10X)   PF00011-C

150 mL



Storage

4°C

Expiration Date

6 months after receiving.

Cited in Article as

PF00011, Foxp3 / Transcription Factor Staining Buffer Kit, Proteintech, IL, USA