CoraLite® Plus 488 Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a, kappa


Mouse and More (1)




CoraLite® Plus 488 Fluorescent Dye



Cat no : CL488-65069


BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

Immunofluorescence (IF)IF : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 3 publications below

Product Information

CL488-65069 targets CD8a in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivityhuman
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Spleen cells or thymocyte membranes
Full Name CD8 antigen, alpha chain
GenBank accession numberBC030679
Gene symbol Cd8a
Gene ID (NCBI) 12525
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).


Product Specific Protocols
IF protocol for CL Plus 488 CD8a antibody CL488-65069Download protocol
Standard Protocols
Click here to view our Standard Protocols



J Immunother Cancer

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Authors - Chushu Li

Mol Cell Endocrinol

Melatonin enhances anti-tumor immunity by targeting macrophages PD-L1 via exosomes derived from gastric cancer cells

Authors - Kaifang Wang

Front Chem

Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency

Authors - Yinghui Shang