Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

Unconjugated

Publications

4

CloneNo.

53-6.7

Cat no : 65069-1-Ig

Synonyms

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

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Validation Data Gallery

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  FC experiment of C57BL/6 mouse splenocytes using 65069-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD8a (65069-1-Ig, Clone:53-6.7) or 0.5 ug Rat IgG2a Isotype Control (2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 2 publications below

Product Information

65069-1-Ig targets CD8a in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Spleen cells or thymocyte membranes
• Full Name: CD8 antigen, alpha chain
• GenBank Accession Number: BC030679
• Gene Symbol: Cd8a
• Gene ID (NCBI): 12525
• RRID: AB_2881470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Borate buffered saline, pH 8.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publications

Species	Application	Title
mouse	FC	Malar J; Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173.; Authors - Huajing Wang
mouse	FC	Nanoscale Adv; Bacteria-mediated tumor immunotherapy via photothermally-programmed PD1 expression; Authors - Wenxuan Xu
mouse	IF	CNS Neurosci Ther; CD8+ T cell infiltration and proliferation in the brainstem during experimental cerebral malaria; Authors - Jun Wang
mouse		Cell Commun Signal; Tumorous IRE1α facilitates CD8+T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma; Authors - Yuqi Yang