CoraLite® Plus 647 Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for IF, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

53-6.7

Cat no : CL647-65069

Synonyms

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

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Validation Data Gallery

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  IF Staining of mouse splenocytes using CL647-65069

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD8a (CL647-65069, Clone: 53-6.7) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL647-65069

  1X10^6 mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD8a (CL647-65069, Clone:53-6.7) or CoraLite® Plus 647-Conjugated rat IgG2a isotype control. Cells were not fixed.

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Tested Applications

• Positive IF detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65069 targets CD8a in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Spleen cells or thymocyte membranes
• Full Name: CD8 antigen, alpha chain
• GenBank Accession Number: BC030679
• Gene Symbol: Cd8a
• Gene ID (NCBI): 12525
• RRID: AB_2883664
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).