Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65069 targets CD8a in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Spleen cells or thymocyte membranes Predict reactive species |
| Full Name | CD8 antigen, alpha chain |
| GenBank Accession Number | BC030679 |
| Gene Symbol | Cd8a |
| Gene ID (NCBI) | 12525 |
| RRID | AB_2883759 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P01731 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer, while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD8a antibody FITC-65069 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse CD8a (53-6.7), catalog number FITC-65069, has 15 citations spanning journals including Nature Communications, Theranostics, Cell Reports, Oncogene, Acta Biomaterialia, and Advanced Healthcare Materials. The research fields primarily involve cancer immunotherapy, vaccine development, immune cell characterization, and tumor microenvironment studies, with flow cytometry as the dominant application for identifying CD8+ T cells in mouse models.
| Species | Application | Title |
|---|---|---|
Nat Commun An engineered linear cap-independent mRNA vaccine with intrinsic adjuvanticity induces potent anti-tumor immunity in mice. | ||
MedComm (2020) Harnessing Tumor-Specific Transcript Diversity Uncovers a Shared Neoantigen Reservoir for Pancreatic Ductal Adenocarcinoma | ||
Adv Healthc Mater Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization | ||
Acta Biomater Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment | ||
Oncogene ZG16 enhances the maturation of dendritic cells via induction of CD40 and contributes to the antitumor immunity in pancreatic cancer |
Reviews
What customers say
One reviewer (Raquel Almeida, April 2026) rated FITC-65069 five stars after using it on mouse blood cells at a 1:200 dilution for both immunofluorescence and flow cytometry. She reported clear, specific staining with low background and strong signal, and recommended the product. Overall, the single review reflects a positive experience with reliable performance across two applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Raquel (Verified Customer) (04-16-2026) | The anti-mouse CD8 antibody worked very well for both immunofluorescence and flow cytometry applications. The staining was clear and specific, with low background and strong signal. I would recommend this product.
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