FITC Plus Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

Publications

5

CloneNo.

53-6.7

Cat no : FITC-65069

Synonyms

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

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Validation Data Gallery

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  FC experiment of mouse splenocytes using FITC-65069

  1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and 0.5 ug FITC Plus Rat IgG2a isotype control (left) or 0.5 ug FITC Plus Anti-Mouse CD8a (FITC-65069, Clone: 53-6.7) (right). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 5 publications below

Product Information

FITC-65069 targets CD8a in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Spleen cells or thymocyte membranes
• Full Name: CD8 antigen, alpha chain
• GenBank Accession Number: BC030679
• Gene Symbol: Cd8a
• Gene ID (NCBI): 12525
• RRID: AB_2883759
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publications

Species	Application	Title
mouse	FC	ACS Appl Mater Interfaces; Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer; Authors - Shichao Zhang
mouse	FC	Adv Healthc Mater; Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization; Authors - Hongping Wan
mouse	FC	Int J Biol Macromol; Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunization; Authors - Kai Deng
	FC	Cancer Immunol Immunother; A nanotherapeutic system for gastric cancer suppression by synergistic chemotherapy and immunotherapy based on iPSCs and DCs exosomes; Authors - Yezhou Li
mouse	FC	Acta Biomater; Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment; Authors - Zhuo Cheng