Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65069 targets CD8a in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Spleen cells or thymocyte membranes Predict reactive species |
| Full Name | CD8 antigen, alpha chain |
| GenBank Accession Number | BC030679 |
| Gene Symbol | Cd8a |
| Gene ID (NCBI) | 12525 |
| RRID | AB_2883759 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P01731 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer, while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD8a antibody FITC-65069 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FITC-65069 antibody (Anti-Mouse CD8a, clone 53-6.7) has 16 total citations. These appear in journals including Nature Communications, Theranostics, MedComm, Advanced Healthcare Materials, Acta Biomaterialia, Oncogene, International Journal of Biological Macromolecules, ACS Applied Materials & Interfaces, Cell Reports, Cancer Immunology & Immunotherapy, Biology Direct, Microorganisms, Journal of Pharmaceutical and Biomedical Analysis, 3 Biotech, Journal of Molecular Neuroscience, and Molecular Cell. Research fields span cancer immunotherapy, vaccine development, immunology, autoimmune encephalitis, and psoriasis.
| Species | Application | Title |
|---|---|---|
Nat Commun An engineered linear cap-independent mRNA vaccine with intrinsic adjuvanticity induces potent anti-tumor immunity in mice. | ||
MedComm (2020) Harnessing Tumor-Specific Transcript Diversity Uncovers a Shared Neoantigen Reservoir for Pancreatic Ductal Adenocarcinoma | ||
Adv Healthc Mater Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization | ||
Acta Biomater Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment | ||
Oncogene ZG16 enhances the maturation of dendritic cells via induction of CD40 and contributes to the antitumor immunity in pancreatic cancer |
Reviews
What customers say
The FITC-65069 anti-mouse CD8 antibody received a 5-star rating from a researcher who used it for both immunofluorescence and flow cytometry on mouse blood cells at a 1:200 dilution. The reviewer reported that the antibody performed very well, producing clear and specific staining with low background and strong signal. Overall, the customer was highly satisfied and recommended the product for these applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Raquel (Verified Customer) (04-16-2026) | The anti-mouse CD8 antibody worked very well for both immunofluorescence and flow cytometry applications. The staining was clear and specific, with low background and strong signal. I would recommend this product.
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