1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.
1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.
FC experiment of human PBMCs using APC-65187
1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.
1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.
Product Information
APC-65187 targets CD68 in FC (Intra) applications and shows reactivity with Human samples.
1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.
FC experiment of human PBMCs using APC-65187
1X10^6 human PBMCs were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), incubated with FC block, then stained with 10 ul APC Anti-Human CD68 (APC-65187, Clone:Y1/82A), or APC Mouse IgG2b Isotype Control. Monocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
