CoraLite® Plus 750 Anti-Human CD68 (Y1/82A)

CD68 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

Y1/82A

Cat no : CL750-65187

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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Validation Data Gallery

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  FC experiment of human PBMCs using CL750-65187

  1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 750 Anti-Human CD68 (CL750-65187, Clone: Y1/82A) or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

• 

  FC experiment of human PBMCs using CL750-65187

  1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 750 Anti-Human CD68 (CL750-65187, Clone:Y1/82A) or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65187 targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Lysosomal contents of lung macrophages
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.